The application of integrated microbioreactor systems is rapidly becoming of more interest to accelerate strain characterization and bioprocess development. However, available high‐throughput screening capabilities are often limited to target extracellular compounds only. Consequently, there is a great demand for automated technologies allowing for miniaturized and parallel cell disruption providing access to intracellular measurements. In this study, a fully automated bead mill workflow was developed and validated for four different industrial platform organisms: Escherichia coli, Corynebacterium glutamicum, Saccharomyces cerevisiae, and Aspergillus niger. The workflow enables up to 48 parallel cell disruptions in microtiter plates and is applicable at‐line to running lab‐scale cultivations. The resulting cell extracts form the basis for quantitative omics studies where no rapid metabolic quenching is required (e.g., genomics and proteomics).

中文翻译：

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通过小型化和自动化珠磨机并行破坏原核和真核细胞

集成微生物反应器系统的应用正在迅速成为加速菌株表征和生物过程开发的兴趣。然而，可用的高通量筛选能力通常仅限于靶向细胞外化合物。因此，对自动化技术有很大的需求，允许小型化和并行细胞破坏，提供细胞内测量。在本研究中，针对四种不同的工业平台生物开发并验证了全自动珠磨机工作流程：大肠杆菌、谷氨酸棒杆菌、酿酒酵母和黑曲霉。该工作流程可在微量滴定板中进行多达 48 次平行细胞破碎，并且适用于在线运行实验室规模的培养。