Superovulation is an important animal breeding biotechnology, while the quality of embryos obtained from superovulation is unstable in cattle. The relationship between the microorganisms in the cattle uterus and embryo qualities was determined to identify the key bacterial populations affecting early embryonic development. A total of 10 Xia Nan cows underwent superovulation, we collected cervical mucus and flush samples to investigated by 16S rDNA sequencing. Results showed that there were abundant microorganisms in cervical mucus, but no obvious relationship with the quality of embryos. The clustering results of flush samples were consistent with the grouping of embryo quality. Proteobacteria accounted for more than 95% of the total bacterial community in group A with the best embryo quality (qualified embryo ratio above 0.8), and as embryo quality decreased, the Proteobacteria proportion also decreased. In contrast to the proportion of Proteobacteria, the proportions of Firmicutes and Bacteroidetes significantly increased as embryo quality decreased. For group C with the worst embryo quality, the proportions of Firmicutes and Bacteroidetes increased to 4.7 times and 12.3 times of group A, respectively. These results showed that the quantities and proportions of Firmicutes and Bacteroidetes may be related to early embryonic development in cattle.

中文翻译：

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鉴定影响牛子宫早期胚胎发育的关键细菌种群。

超数排卵是一项重要的动物育种生物技术，而牛超数排卵获得的胚胎质量不稳定。确定了牛子宫中微生物与胚胎质量之间的关系，以确定影响早期胚胎发育的关键细菌种群。共有 10 头夏南奶牛超排卵，我们采集宫颈粘液和冲洗液样本进行 16S rDNA 测序研究。结果表明，宫颈粘液中微生物含量丰富，但与胚胎质量无明显关系。冲洗样品的聚类结果与胚胎质量的分组一致。A组变形菌占细菌群落总数的95%以上，胚胎质量最好（合格胚胎率在0.8以上），随着胚胎质量的下降，变形菌的比例也随之下降。与变形菌门的比例相反，随着胚胎质量的下降，厚壁菌门和拟杆菌门的比例显着增加。胚胎质量最差的C组，厚壁菌门和拟杆菌门的比例分别增加到A组的4.7倍和12.3倍。这些结果表明，厚壁菌门和拟杆菌门的数量和比例可能与牛的早期胚胎发育有关。分别是A组的3次。这些结果表明，厚壁菌门和拟杆菌门的数量和比例可能与牛的早期胚胎发育有关。分别是A组的3次。这些结果表明，厚壁菌门和拟杆菌门的数量和比例可能与牛的早期胚胎发育有关。