The recent development of next‐generation sequencing technologies has offered valuable insights into individual cells. This technology is centered on the characterization of single cells for epigenomics, genomics, and transcriptomics. Ever since the first report appeared in 2009, the single‐cell RNA‐sequencing saga started to explore deeper into the mechanics intrigued within a single cell. microRNA (miRNA) has been increasingly recognized as an essential molecule triggering an additional layer for gene regulation. Therefore, single‐cell sequencing of miRNAs is crucial to explore the logical riddles surrounding the epigenomics, genomics, and transcriptomics of an individual cell. Scientists from the Vienna Biocenter Campus have lately performed single‐cell sequencing of miRNAs in the fly, Drosophila, and nematode, Caenorhabditis elegans. In this review, we present the latest scientific explorations supported by all‐inclusive data on this novel subject matter of next‐generation sequencing.

中文翻译：

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miRNA的单细胞测序：一项改良技术。

下一代测序技术的最新发展为单个细胞提供了宝贵的见解。这项技术集中于表观基因组学，基因组学和转录组学的单细胞表征。自2009年第一份报告发表以来，单细胞RNA测序的传奇故事就开始更深入地探索单个细胞内的机制。microRNA（miRNA）已被越来越多地认为是触发基因调控附加层的必需分子。因此，miRNA的单细胞测序对于探索围绕单个细胞的表观基因组学，基因组学和转录组学的逻辑难题至关重要。维也纳生物中心校区的科学家最近对果蝇果蝇中的miRNA进行了单细胞测序和线虫，秀丽隐杆线虫。在这篇综述中，我们介绍了有关新一代测序这一新颖主题的全包数据支持的最新科学探索。