Abstract The goal of this work was to establish a method to identify and quantify the primary active components in Angelicae pubescentis Radix quickly, simply, and accurately. This article first reported a high-performance liquid chromatography (HPLC) method that simultaneously determined and quantified osthol, isoimperatorin, and columbianadin using a quantitative analysis of multi-components by single marker (QAMS) method which uses internal reference substances and relative correction factors to perform the synchronous determination and quantitation of multiple components. Taking into account the price and availability of standard substances, osthol was selected to be the internal reference substance for analysis. The compatibility results showed that the relative correction factors of all compounds showed good reproducibility under different chromatographic conditions. In addition, the external standard method (ESM) and QAMS were used to determine and quantify the content of A. pubescentis Radix from nine distinct habitats and in a Chinese patent medicine. The results showed that there were no significant differences between the two methods. In summary, the proposed quantitative QAMS methodology is an alternative approach for the quality control of A. pubescentis Radix.

中文翻译：

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高效液相色谱法 (HPLC) 使用单标记物 (QAMS) 校准法对多组分进行定量分析，同时测定当归中的蛇床子素、蒺藜苷和异欧前胡素

摘要 本工作的目的是建立一种快速、简单、准确地鉴定和定量当归中主要活性成分的方法。本文首次报道了一种高效液相色谱 (HPLC) 方法，该方法使用单标记物 (QAMS) 方法对多组分进行定量分析，该方法使用内标物质和相对校正因子来同时测定和定量蛇床素、异欧前胡素和哥伦比亚丁。执行多种组分的同步测定和定量。考虑到标准物质的价格和可获得性，选择雌二醇作为分析的内标物质。相容性结果表明，所有化合物的相对校正因子在不同色谱条件下均表现出良好的重现性。此外，外标法（ESM）和QAMS被用于测定和量化来自九个不同产地和中成药中的A. pubescentis Radix的含量。结果表明，两种方法之间没有显着差异。总之，所提出的定量 QAMS 方法是一种用于控制青春痘质量的替代方法。结果表明，两种方法之间没有显着差异。总之，所提出的定量 QAMS 方法是一种用于控制青春痘质量的替代方法。结果表明，两种方法之间没有显着差异。总之，所提出的定量 QAMS 方法是一种用于控制青春痘质量的替代方法。