Abstract

The present study deals with Plectranthus amboinicus (Lour.), multiple potential herbs perceived as a medicinal and culinary adjunct has been used to fabricate gold (Au) nanoparticles (NPs) in an eco-benign fashion. We have primly reported the antioxidant and anticarcinogenic effects of phytofabricated Au NPs towards lung adenocarcinoma (LAC) cells (A549 cell line) in vitro. The herbal concentrate, size and dosage of Au NPs were the key variables that determined the anticarcinogenic efficacy. Results indicated that the herbal concentrate encompassing flavonoids, alkaloids and polyphenolics aided reduction and stabilisation of Au NPs supported by spectral (λ_max @ 550 nm) and quantitative analyses. The Fourier transform-infrared spectra anticipated distinct phenolics and protein frequencies involved in Au NPs capping in the course of fabrication. The manipulated Au NPs of 15 nm size has significantly influenced the radical scavenging (48.1 μg) and proliferation pattern of LAC cells (A549) associated with cytotoxic characteristics and IC₅₀ (Au NPs concentration linked with 50% mortality) defined at 16.3 μg/mL in 48 h. This nanotoxicological prospect on the use of nanomaterials as an anticancer agent would be an alternative for the emerging intrinsic resistance of cells to the drug; a great challenge pertaining to safer environmental and biological outcomes.

摘要

本研究涉及Plectranthus amboinicus（Lour。），人们已将多种潜在的草药作为药用和烹饪助剂，以生态良性的方式制备了金（Au）纳米颗粒（NP）。我们正色报道的抗氧化和phytofabricated金纳米粒子对肺腺癌（LAC）细胞（A549细胞系）抗癌作用体外。草药浓缩物，金纳米颗粒的大小和剂量是决定抗癌功效的关键变量。结果表明，所述草药浓缩物包含类黄酮，生物碱和多酚辅助金纳米粒子的还原和稳定化通过光谱（支持的λ_最大550 nm）和定量分析。傅里叶变换红外光谱预测了在制造过程中金NP封端所涉及的酚和蛋白质的频率不同。15 nm大小的可操纵Au NPs显着影响LAC细胞（A549）的自由基清除（48.1μg）和增殖模式，与细胞毒性特征和IC ₅₀（Au NPs浓度与50％死亡率有关）定义为16.3μg/ mL在48小时内 使用纳米材料作为抗癌剂的这种纳米毒理学前景将替代细胞对药物产生的内在抗性。与更安全的环境和生物结果有关的巨大挑战。