Microalgal biomass is composed of different valuable metabolites that can satisfy the requirements of renewable biofuels, alternative proteins, carbohydrates, and food grade natural colorants. Production of a specific product from microalgae has been proved to be economically infeasible on the commercial scale except for the production of high-value products (e.g. carotenoids and phycobiliproteins). Therefore, the simultaneous extraction of multiple products is essential to bring pragmatism for the production of biofuels, proteins, and carbohydrate derived products from microalgal biomass. In order to obtain multiple products, various strategies have been implemented using potential techniques of cell disruption and biomass fractionation based on the priorities of products. Conventional approaches of downstream processing have often proved to be inefficient in the case of integrated fractionation systems. This is attributable to the divergent nature of the intracellular metabolites of microalgae and their vulnerability toward the different chemicals and conditions of those downstream processes. However, three phase partitioning (TPP), aqueous two-phase separation, membrane separation, supercritical fluid extraction (SFE), and pressurized liquid extraction (PLE) are some of the advanced techniques which have been proved to be useful in this regard. Choice of cell disruption mechanisms is critical for several purposes, such as the selective release of metabolites into a suitable solvent, preservation of bioactivity of molecules and cost-savings. Unfortunately, consolidated report for the fractionation of priority-based products from microalgal biomass using these techniques is lacking. Therefore, in this review, we have critically discussed the different strategies for the priority-based multiple products by implementation of the advanced techniques.

中文翻译：

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来自微藻的基于优先级的多种产品：技术和策略回顾。

微藻生物质由各种有价值的代谢物组成，可以满足可再生生物燃料，替代蛋白，碳水化合物和食品级天然着色剂的需求。从微藻类生产特定产品在商业规模上已被证明在经济上是不可行的，除了生产高价值产品（如类胡萝卜素和藻胆蛋白）外。因此，多种产品的同时提取对于将实用主义用于从微藻生物质生产生物燃料，蛋白质和碳水化合物衍生产品至关重要。为了获得多种产物，已经基于产物的优先级使用潜在的细胞分裂和生物质分级技术来实施各种策略。在集成分馏系统的情况下，常规的下游加工方法通常被证明是无效的。这归因于微藻细胞内代谢产物的不同性质，以及它们对那些下游过程的不同化学物质和条件的脆弱性。但是，三相分配（TPP），水两相分离，膜分离，超临界流体萃取（SFE）和加压液体萃取（PLE）是一些先进的技术，已被证明在这方面有用。对于多种目的而言，细胞破坏机制的选择至关重要，例如将代谢物选择性释放到合适的溶剂中，保持分子的生物活性和节省成本。不幸，缺乏使用这些技术从微藻生物质中分离优先产品的综合报告。因此，在这篇综述中，我们通过实施先进技术来严格讨论基于优先级的多种产品的不同策略。