BACKGROUND ACS (acute coronary syndrome), a subgroup of coronary artery disease (CHD), is a leading cause of death worldwide. Reports shown the association between methylation and CHD, while the abnormal expression of C1QTNF1 (C1q and tumor necrosis factor related protein 1) in CHD patients, but the underlying mechanisms are still unclear. OBJECTIVE To analyze the methylation of CpG sites of C1QTNF1 in ACS patients. METHODS Peripheral blood samples were collected from healthy controls and ACS patients. The methylation of total C1QTNF1, promoter sequence and CpG sites of C1QTNF1 were measured using methylation detection kits. The outcomes were compared between patients and controls based on gender, clinical classification and clinical stages. RESULTS The promoter sequences from 37 ACS patients and 20 controls indicate that the methylation rate of C1QTNF1 was significantly lower in male patients compared to healthy controls at + 63 CpG sites (p = 0.03). Whereas, the methylation rate of C1QTNF1 in female patients was significantly lower than female health controls at - 89, + 39 and + 167 CpG sites (p = 0.021, 0.042, 0.021). In addition, the methylation rate of C1QTNF1 was significantly higher in male patients than female patients at - 89, - 41 and + 39 CpG sites (p = 0.011, 0.043, 0.006). Moreover, the methylation rate significantly decreased at - 24 sites (p = 0.021), but it significantly increased at - 14 site (p = 0.048) in patients with UA, compared to patients with STEMI (ST-segment elevation myocardial infarction). CONCLUSIONS There were significant differences in the methylation rate + 63 CpG sites between controls and male ACS patients. The - 14 site methylation increased in patients with UA, compared to patients with STEMI.

中文翻译：

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汉族人群急性冠脉综合征中C1QTNF1（C1q和肿瘤坏死因子相关蛋白1）中CpG位点的甲基化存在性别差异：病例对照研究。

背景技术ACS（急性冠状动脉综合征）是冠状动脉疾病（CHD）的一个亚类，是世界范围内主要的死亡原因。有报告显示甲基化与冠心病之间存在关联，而冠心病患者中C1QTNF1（C1q和肿瘤坏死因子相关蛋白1）的异常表达，但其潜在机制仍不清楚。目的分析ACS患者C1QTNF1 CpG位点的甲基化。方法从健康对照者和ACS患者中采集外周血。使用甲基化检测试剂盒测量总C1QTNF1的甲基化，C1QTNF1的启动子序列和CpG位点。根据性别，临床分类和临床阶段，比较患者和对照组的结局。结果来自37位ACS患者和20位对照的启动子序列表明，与+63 CpG位点的健康对照相比，男性患者C1QTNF1的甲基化率显着更低（p = 0.03）。而在-89，+ 39和+ 167 CpG位点，女性患者中C1QTNF1的甲基化率显着低于女性健康对照者（p = 0.021、0.042、0.021）。此外，在-89，-41和+ 39个CpG位点，男性患者中C1QTNF1的甲基化率明显高于女性患者（p = 0.011、0.043、0.006）。而且，与STEMI患者（ST段抬高型心肌梗死）相比，UA患者的甲基化率在-24个位点显着降低（p = 0.021），但在-14个位点（p = 0.048）显着增加。结论对照组和男性ACS患者之间的甲基化率+ 63 CpG位点存在显着差异。与STEMI患者相比，UA患者的-14位甲基化增加。