Due to global warming, high temperature has become the main abiotic stress affecting plant growth worldwide. LysM-containing receptor-like kinase 4 (LYK4) is the receptor for chitin, and ERECTA(ER) is a key factor in plant tolerance to high temperature. In this study, we constructed a chitin-induced chimeric LYK4-ER gene, in which the extracellular region and transmembrane domain of the LYK4 gene are fused with the intracellular region of the ER gene. Colony PCR, RT-PCR and western blot analyses of LYK4-ER transcription in plants, confirmed that the LYK4-ER gene was successfully constructed and transferred into Arabidopsis. The LYK4-ER gene localized to the cytomembrane and cytoplasm in vivo because of the binding properties of the transmembrane domain of the LYK4-ER gene to the cell membrane. The transgenic plants showed a higher germination rate and germination index as well as a shorter mean germination time than the wild-type plants, indicating that the LYK4-ER gene increases the heat tolerance of Arabidopsis. The lower H₂O₂ content and relative electrolytic leakage of the transgenic plants showed that the status of these plants under heat stress was improved. UPLC-MS/MS was used to analyze the phytohormones content, which suggested that the transgenic plants exhibited improved heat tolerance through jasmonic acid signal transduction pathways.

中文翻译：

---

几丁质诱导的嵌合LYK4-ER基因提高了拟南芥幼苗期的耐热性。

由于全球变暖，高温已成为影响全球植物生长的主要非生物胁迫。含有LysM的受体样激酶4 （LYK4）是几丁质的受体，而ERECTA（ER）是植物对高温的耐受性的关键因素。在这项研究中，我们构建了几丁质诱导的嵌合LYK4 - ER基因，其中LYK4基因的细胞外区域和跨膜结构域与ER基因的细胞内区域融合。对植物中LYK4-ER转录的菌落PCR，RT-PCR和western blot分析，证实了LYK4 - ER该基因已成功构建并转移到拟南芥中。由于LYK4 - ER基因的跨膜结构域与细胞膜的结合特性，LYK4 - ER基因在体内定位于细胞膜和细胞质。与野生型植物相比，转基因植物显示出更高的发芽率和发芽指数以及更短的平均发芽时间，表明LYK4 - ER基因提高了拟南芥的耐热性。较低的H ₂ O ₂转基因植物的含量和相对电解渗漏表明，这些植物在热胁迫下的状况得到改善。用UPLC-MS / MS分析植物激素含量，表明转基因植物通过茉莉酸信号转导途径表现出提高的耐热性。