The regeneration from embryogenic callus of higher plants in tissue culture is regulated by explants types and developmental stage and also regulated by some genes. In Miscanthus lutarioriparius, five candidate genes were selected to decide the differential expression between embryogenic and non-embryogenic calli, including MlARF-GEP (guanine nucleotide-exchange protein of ADP ribosylation factor), MlKHCP (kinesin heavy chain like protein), MlSERK1 (somatic embryogenesis receptor-like kinases 1), MlSERK2 (somatic embryogenesis reportor-like kinases 2), and MlTypA (tyrosine phosphorylation protein A) with Genbank accession numbers KU640196–KU640200. Multiple sequence alignment analysis showed that five genes were highly conserved among members of their gene families respectively. Phylogenetic relationship analysis showed that five genes were closest with homologous genes of Zea mays and Sorghum. The qRT-PCR results showed significant differences of five genes expression pattern between two different callus types, the relative expression in embryogenic callus was detected to exceed in non-embryogenic callus. Furthermore, simple sequence repeats (SSR) marker statistics results via Chi-square showed a significant correlation between MlSERK1 genotype and induction of embryogenic callus in M. lutarioriparius. This study may lay the foundation of the molecular mechanism on the embryogenic callus induction of M. lutarioriparius and perhaps provide some gist for further study on genetic manipulation.

组织培养中高等植物胚发生愈伤组织的再生受外植体类型和发育阶段的调节，也受某些基因的调节。在Miscanthus lutarioriparius中，选择了五个候选基因来决定胚性和非胚性愈伤组织之间的差异表达，包括MlARF-GEP（ADP核糖基化因子的鸟嘌呤核苷酸交换蛋白），MlKHCP（驱动蛋白重链样蛋白），MlSERK1（体细胞）胚胎发生受体样激酶1），MlSERK2（体细胞胚胎发生报告基因样激酶2）和MlTypAGenbank登录号KU640196–KU640200的（酪氨酸磷酸化蛋白A）。多重序列比对分析表明，五个基因在其基因家族成员中分别是高度保守的。系统发育关系分析表明，5个基因与玉米和高粱的同源基因最接近。qRT-PCR结果显示两种不同类型愈伤组织之间五个基因表达模式的显着差异，检测到胚性愈伤组织中的相对表达超过非胚性愈伤组织。此外，简单序列重复通过卡方（SSR）标记的统计结果显示之间的相关性显著MlSERK1基因型和在胚发生愈伤组织的诱导M. lutarioriparius。这项研究可能为lutarioriparius胚发生愈伤组织诱导奠定分子机制的基础，并可能为进一步研究遗传操纵提供一些依据。