MicroRNAs (miRNAs) are highly conserved, endogenous small RNAs that regulate gene expression at the post-transcriptional level. miR-127 plays an essential role in myogenic differentiation in vivo and in vitro. However, it is not clear whether miR-127-3p affects myogenic cell proliferation. The detailed function of miR-127-3p in proliferative C2C12 cell lines and further identified its regulatory mechanism by qRT-PCR, western blot, flow cytometry analysis and luciferase reporter assay. Overexpression of miR-127-3p significantly inhibited proliferation of C2C12 cells and vice versa. Sept7 was a target gene of miR-127-3p using dual-luciferase reporter assay, qRT-PCR, and western blotting. The RNA interference analysis, in which Sept7 was downregulated, showed that Sept7 significantly promoted the proliferation of C2C12 cells. Besides, the expression level of Sept7 was detected analysis in muscle cells and tissues. These findings reveal that miR-127-3p regulates myoblast proliferation by targeting Sept7.

中文翻译：

---

MicroRNA-127-3p 通过靶向 Sept7 调节成肌细胞增殖

MicroRNA (miRNA) 是高度保守的内源性小 RNA，可在转录后水平调节基因表达。miR-127 在体内和体外的肌源性分化中起重要作用。然而，尚不清楚miR-127-3p是否影响生肌细胞增殖。miR-127-3p 在增殖性 C2C12 细胞系中的详细功能，并通过 qRT-PCR、蛋白质印迹、流式细胞术分析和荧光素酶报告基因测定进一步确定其调节机制。miR-127-3p的过表达显着抑制了C2C12细胞的增殖，反之亦然。Sept7 是使用双荧光素酶报告基因检测、qRT-PCR 和蛋白质印迹的 miR-127-3p 的靶基因。RNA干扰分析，其中 Sept7 下调，表明 Sept7 显着促进 C2C12 细胞的增殖。除了，在肌肉细胞和组织中检测分析 Sept7 的表达水平。这些发现表明 miR-127-3p 通过靶向 Sept7 调节成肌细胞增殖。