Biodiesel is an alternative to fossil diesel, non-toxic, and less pollutant. The production of biodiesel occurs with the use of oils, which are extracted from oleaginous seeds such as soybean, rapeseed, and palm fruit. The extraction of oils from oilseeds is one bottleneck of biodiesel production impacting on significant processes’ losses and productivity. This review analyzes different technologies to improve the oil extraction focusing on the enzyme-assisted aqueous extraction (EAAE) methods. EAAE is an environmentally friendly technology that takes advantage of the degradation efficiency of the enzymes, in this case hemicellulases preparations, which specifically degrade different structures that are present in vegetable cell walls to improve oil extraction from oilseeds. The enzymes used in this process are industrially produced and are highly efficient, but with high costs. The use of agro-industrial subproducts or the improvement of the enzymes’ producing strains could be an interesting solution for viable enzymatic preparations’ production to attend the biodiesel industries’ demand. EAAE treatment also shows great potential in techno-economic analysis allowing a faster recovery of profits and at a smaller production scale than the hexane extraction methodology.

生物柴油是化石柴油的替代品，无毒，污染少。生物柴油的生产需要使用油，这些油是从油质种子（例如大豆，油菜籽和棕榈果）中提取的。从油料籽中提取油是生物柴油生产的一个瓶颈，会严重影响工艺的损失和生产率。这篇综述分析了不同的技术来改善油的提取，重点是酶辅助水提取（EAAE）方法。EAAE是一项环保技术，利用酶的降解效率（在本例中为半纤维素酶制剂）来专门降解植物细胞壁中存在的不同结构，以改善从油料种子中提取油的能力。在该方法中使用的酶是工业生产的并且是高效的，但是成本高。农用副产物的使用或酶生产菌株的改进可能是可行的酶制剂生产的有趣解决方案，以满足生物柴油行业的需求。EAAE处理在技术经济分析中也显示出巨大的潜力，与己烷萃取方法相比，可以更快地回收利润并以较小的生产规模。