The great expansion in recent years in the use of plant biostimulants has underestimated the possibility of producing environmental risks. The objective of this study was to evaluate the cytotoxicity, genotoxicity, and toxicity of three different biostimulants (A, B, and C) produced and widely marketed in Brazil and exported to other countries on both plant and animal indicator organisms. In Allium cepa (non-target species of these plant biostimulants), the concentrations evaluated for the products A and B (0.0625 at 0.500 mL/L), and C (0.0625 at 0.500 g/L) induced inhibition of cell proliferation on meristems, in at least one time of analysis, characterizing them as cytotoxic. The three biostimulants promoted an expressive number of prophases in detriment to the other phases of cell division. In the test with Artemia salina, different concentrations of the biostimulants were tested, ranging from 1000 to 1.95 ppm, and the toxic potential was evaluated through the LC₅₀. The three products showed high toxicity (LC₅₀ < 100 ppm) against microcrustaceans. The results obtained in the present study suggest that the evaluated biostimulants may be toxic to non-target plants and toxic to organisms in water bodies influenced by leaching from soil with the presence of these substances.

中文翻译：

---

巴西生产的植物生物刺激剂的细胞毒性，遗传毒性和毒性：确定非目标物种环境风险的补贴

近年来，植物生物兴奋剂的使用大大扩展，低估了产生环境风险的可能性。这项研究的目的是评估在巴西生产并广泛销售并出口到其他国家的动植物指示物上的三种不同生物刺激剂（A，B和C）的细胞毒性，遗传毒性和毒性。在葱属（这些植物生物刺激剂的非目标物种），产品A和B（0.500 mL / L时为0.0625）和C（0.500 g / L时为0.0625）评估的浓度，至少在分生组织上诱导了细胞增殖的抑制分析一次，将其表征为具有细胞毒性。三种生物刺激剂促进了前阶段的表达数量，从而损害了细胞分裂的其他阶段。在卤虫卤虫的测试中，测试了不同浓度的生物刺激剂，范围从1000到1.95 ppm，并通过LC ₅₀评估了潜在的毒性。三种产品均显示出高毒性（LC ₅₀ <100 ppm）对抗微甲壳类动物。在本研究中获得的结果表明，所评估的生物刺激剂可能对非目标植物有毒，并且对这些水体中的水体中的有机物有毒，这些物质的存在会影响其从土壤中的浸出。