当前位置: X-MOL 学术Plant Cell Physiol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
A Genome-Wide Association Study for Resistance to the Insect Pest Leptocybe invasa in Eucalyptus grandis Reveals Genomic Regions and Positional Candidate Defense Genes.
Plant & Cell Physiology ( IF 3.9 ) Pub Date : 2020-05-07 , DOI: 10.1093/pcp/pcaa057
Lorraine Mhoswa 1 , Marja M O'Neill 1 , Makobatjatji M Mphahlele 1, 2 , Caryn N Oates 1 , Kitt G Payn 3 , Bernard Slippers 1 , Alexander A Myburg 1 , Sanushka Naidoo 1
Affiliation  

The galling insect, Leptocybe invasa, causes significant losses in plantations of various Eucalyptus species and hybrids, threatening its economic viability. We applied a Genome-Wide Association Study (GWAS) to identify single nucleotide polymorphism (SNP) markers associated with resistance to L. invasa. A total of 563 insect-challenged Eucalyptus grandis trees, from 61 half-sib families, were genotyped using the EUChip60K SNP chip and we identified 15,445 informative SNP markers in the test population. Multi-Locus Mixed Model (MLMM) analysis identified 35 SNP markers putatively associated with resistance to L. invasa based on four discreet classes of insect damage scores: (0)- not infested, (1)- infested showing evidence of oviposition but no gall development, (2)- infested with galls on leaves, mid-ribs or petioles and (3)- stunting and lethal gall formation. MLMM analysis identified three associated genomic regions on chromosomes 3, 7 and 8 jointly explaining 17.6% of the total phenotypic variation. SNP analysis of a validation population of 494 E. grandis trees confirmed seven SNP markers that were also detected in the initial association analysis. Based on transcriptome profiles of resistant and susceptible genotypes from an independent experiment we identified several putative candidate genes in associated genomic loci including NB-ARC and TIR-NBS-LRR genes. Our results suggest that Leptocybe resistance in E. grandis may be influenced by a few large-effect loci in combination with minor effect loci segregating in our test and validation populations.

中文翻译:

全基因组研究对桉树对昆虫害​​虫Leptocybe invasa的抗性揭示了基因组区域和位置候选防御基因。

蠕虫昆虫Leptocybe invasa在各种桉树种和杂种的人工林中造成重大损失,威胁到其经济生存能力。我们应用了全基因组关联研究(GWAS)来确定与对L. invasa的抗性相关的单核苷酸多态性(SNP)标记。使用EUChip60K SNP芯片对来自61个半同胞家族的563棵昆虫激发大桉树进行了基因分型,我们在测试群体中鉴定了15,445个信息丰富的SNP标记。多基因座混合模型(MLMM)分析基于四种谨慎的昆虫损害评分类别,确定了35种SNP标记可能与对侵袭乳杆菌的抗性相关:(0)-未侵染,(1)-侵染显示产卵但无胆汁发育,(2)-叶子上长有虫s,中肋骨或叶柄和(3)发育迟缓和致死性胆汁形成。MLMM分析确定了染色体3、7和8上的三个相关基因组区域,共同解释了总表型变异的17.6%。对494棵大树的验证种群进行SNP分析,确认了在初始关联分析中也检测到的七个SNP标记。基于来自独立实验的抗性和易感基因型的转录组图谱,我们在相关的基因组位点中鉴定了几个推定的候选基因,包括NB-ARC和TIR-NBS-LRR基因。我们的结果表明,在我们的测试和验证人群中,E。grandis的Leptocybe耐药性可能受一些大影响基因座与次要影响基因座分离的影响。MLMM分析确定了染色体3、7和8上的三个相关基因组区域,共同解释了总表型变异的17.6%。对494棵大树的验证种群进行SNP分析,确认了在初始关联分析中也检测到的7个SNP标记。基于来自独立实验的抗性和易感基因型的转录组图谱,我们在相关的基因组位点中鉴定了几个推定的候选基因,包括NB-ARC和TIR-NBS-LRR基因。我们的结果表明,在我们的测试和验证人群中,E。grandis的Leptocybe耐药性可能受几个大影响基因座与次要影响基因座分离的影响。MLMM分析确定了染色体3、7和8上的三个相关基因组区域,共同解释了总表型变异的17.6%。对494棵大树的验证种群进行SNP分析,确认了在初始关联分析中也检测到的7个SNP标记。基于来自独立实验的抗性和易感基因型的转录组图谱,我们在相关的基因组位点中鉴定了几个推定的候选基因,包括NB-ARC和TIR-NBS-LRR基因。我们的结果表明,在我们的测试和验证人群中,E。grandis的Leptocybe耐药性可能受几个大影响基因座与次要影响基因座分离的影响。大树证实了在初始关联分析中也检测到的七个SNP标记。基于来自独立实验的抗性和易感基因型的转录组图谱,我们在相关的基因组位点中鉴定了几个推定的候选基因,包括NB-ARC和TIR-NBS-LRR基因。我们的结果表明,在我们的测试和验证人群中,E。grandis的Leptocybe耐药性可能受一些大影响基因座与次要影响基因座分离的影响。大树证实了在初始关联分析中也检测到的七个SNP标记。基于来自独立实验的抗性和易感基因型的转录组图谱,我们在相关的基因组位点中鉴定了几个推定的候选基因,包括NB-ARC和TIR-NBS-LRR基因。我们的结果表明,在我们的测试和验证人群中,E。grandis的Leptocybe耐药性可能受几个大影响基因座与次要影响基因座分离的影响。
更新日期:2020-05-07
down
wechat
bug