Phomopsis asparagi is one of the most serious fungal pathogens, which causes stem blight disease in Asparagus officinalis (AO), adversely affecting its production worldwide. Recently, the development of novel asparagus varieties using wild Asparagus genetic resources with natural P. asparagi resistance has become a priority in Japan due to the lack of resistant commercial AO cultivars. In this study, comparative metabolome and transcriptome analyses of susceptible AO and resistant wild Asparagus kiusianus (AK) 24 and 48 h postinoculated (AOI_24 hpi, AOI_48 hpi, AKI_24 hpi and AKI_48 hpi, respectively) with P. asparagi were conducted to gain insights into metabolic and expression changes associated with AK species. Following infection, the resistant wild AK showed rapid metabolic changes with increased levels of flavonoids and steroidal saponins and decreased asparagusic acid glucose ester content, compared with the susceptible AO plants. Transcriptome data revealed a total of 21 differentially expressed genes (DEGs) as the core gene set that displayed upregulation in the resistant AK versus susceptible AO after infection with P. asparagi. Kyoto Encyclopedia of Genes and Genomes pathway analysis of these DEGs identified 11 significantly enriched pathways, including flavonoid biosynthesis and primary metabolite metabolism, in addition to plant signaling and defense-related pathways. In addition, comparative single-nucleotide polymorphism and Indel distributions in susceptible AO and resistant AK plants were evaluated using the latest AO reference genome Aspof.V1. The data generated in this study are important resources for advancing Asparagus breeding programs and for investigations of genetic linkage mapping, phylogenetic diversity and plant defense-related genes.

中文翻译：

---

敏感的芦笋和抗性野生曲霉的比较代谢组和转录组分析揭示了对枯萎病抗性的见解。

芦笋茎枯是最严重的真菌病原体，导致遏制疫病一个芦笋（AO），不利影响其生产全世界。最近，使用野生芦笋新品种的开发芦笋遗传资源与自然P.芦笋性已经成为日本的当务之急是由于缺乏性商业AO品种。在这项研究中，比较敏感的AO和抗性野生天门芦（AKI）分别在接种天冬氨酸后24小时和48小时（AOI_24 hpi，AOI_48 hpi，AKI_24 hpi和AKI_48 hpi）的代谢组和转录组分析。进行了深入了解与AK物种相关的代谢和表达变化。感染后，与易感的AO植物相比，抗性野生AK表现出快速的代谢变化，其中黄酮类和甾体皂苷的含量增加，而天冬酰胺酸葡萄糖酯含量降低。转录组数据显示，共有21个差异表达基因（DEGs）作为核心基因集，在感染天门冬青霉菌后，抗性AK相对于易感AO表现出上调。这些DEG的《京都基因与基因组百科全书》通路分析确定了11种显着丰富的通路，包括黄酮类生物合成和主要代谢产物代谢，以及植物信号传导和防御相关通路。此外，使用最新的AO参考基因组Aspof.V1对易感AO和抗性AK植物中的比较单核苷酸多态性和Indel分布进行了评估。这项研究产生的数据是推进芦笋育种计划和研究遗传连锁作图，系统发育多样性和植物防御相关基因的重要资源。