We recently developed a self-assembling peptide, E1Y9 (Ac-E-YEYKYEYKY-NH 2 ), that forms supramolecular nanofibers with hydrogelation properties in response to Ca 2+ ions. In this study, we proposed a new method to functionalize supramolecular peptide nanofibers using fiber-binding peptides screened from a phage peptide library. Three fiber-binding peptides, namely, p1, p2, and p3, were successfully identified. The RGDS-conjugated forms of these peptides, namely, p1-RGDS, p2-RGDS, and p3-RGDS, significantly enhanced the adhesion of 3T3-L1 cells on E1Y9 nanofibers through noncovalent modification of E1Y9 nanofibers. This noncovalent modification using fiber-binding peptides was also effective for functionalization of E1Y9 hydrogels, as E1Y9 hydrogels modified with p1-RGDS or p2-RGDS promoted the proliferation of 3T3-L1 cells, compared with the E1Y9 hydrogel alone. Since p1 and p2 could bind to different sites on E1Y9 nanofibers, the additional enhancement in cell proliferation on E1Y9 hydrogels was achieved using both p1-RGDS and p2-RGDS. Thus, the functionalization of supramolecular peptide nanofibers using molecular recognition peptides will be a powerful tool for the development of functional materials. We proposed a new method to functionalize supramolecular peptide nanofibers using fiber-binding peptides screened from a phage peptide library. The RGDS-conjugated fiber-binding peptides significantly enhanced the adhesion of 3T3-L1 cells on E1Y9 nanofibers through noncovalent modification of E1Y9 nanofibers. This noncovalent modification using fiber-binding peptides was also effective for functionalization of E1Y9 hydrogels as cell culture materials that promoted the proliferation of 3T3-L1 cells. The functionalization of supramolecular peptide nanofibers using molecular recognition peptides will be a powerful tool for the development of functional materials.

中文翻译：

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利用超分子肽纳米纤维的分子识别功能化自组装肽材料

我们最近开发了一种自组装肽 E1Y9 (Ac-E-YEYKYEYKY-NH 2 )，它可以响应 Ca 2+ 离子形成具有水凝胶特性的超分子纳米纤维。在这项研究中，我们提出了一种使用从噬菌体肽库中筛选出的纤维结合肽功能化超分子肽纳米纤维的新方法。成功鉴定了三种纤维结合肽，即 p1、p2 和 p3。这些肽的 RGDS 偶联形式，即 p1-RGDS、p2-RGDS 和 p3-RGDS，通过对 E1Y9 纳米纤维的非共价修饰，显着增强了 3T3-L1 细胞在 E1Y9 纳米纤维上的粘附。这种使用纤维结合肽的非共价修饰对 E1Y9 水凝胶的功能化也有效，因为用 p1-RGDS 或 p2-RGDS 修饰的 E1Y9 水凝胶促进了 3T3-L1 细胞的增殖，与单独的 E1Y9 水凝胶相比。由于 p1 和 p2 可以与 E1Y9 纳米纤维上的不同位点结合，因此使用 p1-RGDS 和 p2-RGDS 都可以进一步增强 E1Y9 水凝胶上的细胞增殖。因此，使用分子识别肽对超分子肽纳米纤维进行功能化将成为开发功能材料的有力工具。我们提出了一种使用从噬菌体肽库中筛选出来的纤维结合肽功能化超分子肽纳米纤维的新方法。RGDS 结合的纤维结合肽通过对 E1Y9 纳米纤维的非共价修饰显着增强了 3T3-L1 细胞在 E1Y9 纳米纤维上的粘附。这种使用纤维结合肽的非共价修饰对于作为促进 3T3-L1 细胞增殖的细胞培养材料的 E1Y9 水凝胶的功能化也是有效的。利用分子识别肽对超分子肽纳米纤维进行功能化将成为功能材料开发的有力工具。