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Soaking with an essential mineral (Fe, Zn, Cu, Mn and Se) mixture delays senescence and improves nutrient accumulation in postharvest fruit of Ziziphus jujuba
Postharvest Biology and Technology ( IF 6.4 ) Pub Date : 2020-08-01 , DOI: 10.1016/j.postharvbio.2020.111186
Benliang Deng , Haojie Shi , Hongxia Liu , Shipeng Li , Shan Tian , Xusheng Zhao

Abstract Fresh jujube is a healthy climacteric fruit with a high medicinal value. However, it is highly perishable during harvest and storage. In addition, essential minerals (EMs) and antioxidants are indispensable nutrients for human health. In the present study, the effects of an EM mixture (containing 1 mM Fe, Zn, Cu, Mn, and Se) on postharvest jujube fruit senescence, and antioxidant and mineral nutrient accumulation, were investigated. The results showed that soaking with the EM mixture delayed fruit senescence by decreasing the reddening index, coupled with reduced weight loss and decay incidence, and maintained firmness. In addition, greater total antioxidant capacity, as well as higher accumulation of iron, zinc, copper, manganese, selenium and total phenolics, was detected in the EM mixture-treated fruit compared with those in the control. However, the EM-delayed senescence and improved antioxidant capacity could be partly abolished by dimethylthiourea (a specific ROS scavenger) or diphenyleneiodonium chloride (a specific inhibitor of NADPH oxidase (NOX)). Further experiments showed that higher H2O2 levels and NOX activities could be detected in EM-treated fruit compared with those in the control. However, the EM-enhanced H2O2 content and NOX activity were partly attenuated by diphenyleneiodonium chloride. These results showed that the NOX-mediated H2O2 is required for EM-delayed postharvest jujube fruit senescence. Thus, EM soaking provides us a new method to delay postharvest fruit senescence and improve nutrient quality.

中文翻译:

用必需矿物质(Fe、Zn、Cu、Mn 和 Se)混合物浸泡可延缓衰老并改善大枣采后果实的营养积累

摘要 鲜枣是一种健康的更年期水果,具有很高的药用价值。然而,它在收获和储存过程中极易腐烂。此外,必需矿物质 (EM) 和抗氧化剂是人类健康不可或缺的营养素。在本研究中,研究了 EM 混合物(含有 1 mM Fe、Zn、Cu、Mn 和 Se)对采后枣果实衰老以及抗氧化剂和矿物质营养积累的影响。结果表明,用 EM 混合物浸泡可通过降低变红指数来延缓果实衰老,同时减少重量损失和腐烂发生率,并保持果实的硬度。此外,更大的总抗氧化能力,以及更高的铁、锌、铜、锰、硒和总酚类物质的积累,与对照组相比,在 EM 混合物处理的水果中检测到了。然而,二甲基硫脲(一种特定的 ROS 清除剂)或二亚苯基氯化碘(NADPH 氧化酶 (NOX) 的一种特定抑制剂)可以部分消除 EM 延迟衰老和提高的抗氧化能力。进一步的实验表明,与对照相比,经过 EM 处理的水果中可以检测到更高的 H2O2 水平和 NOX 活性。然而,EM 增强的 H2O2 含量和 NOX 活性被二亚苯基氯化物部分削弱。这些结果表明,NOX 介导的 H2O2 是 EM 延迟采后枣果实衰老所必需的。因此,EM浸泡为我们提供了一种延缓果实采后衰老和提高营养品质的新方法。EM 延迟衰老和提高的抗氧化能力可以通过二甲基硫脲(一种特定的 ROS 清除剂)或二亚苯基氯化碘(NADPH 氧化酶 (NOX) 的一种特定抑制剂)部分消除。进一步的实验表明,与对照相比,经过 EM 处理的水果中可以检测到更高的 H2O2 水平和 NOX 活性。然而,EM 增强的 H2O2 含量和 NOX 活性被二亚苯基氯化物部分削弱。这些结果表明,NOX 介导的 H2O2 是 EM 延迟采后枣果实衰老所必需的。因此,EM浸泡为我们提供了一种延缓果实采后衰老和提高营养品质的新方法。EM 延迟衰老和提高的抗氧化能力可以通过二甲基硫脲(一种特定的 ROS 清除剂)或二亚苯基氯化碘(NADPH 氧化酶 (NOX) 的一种特定抑制剂)部分消除。进一步的实验表明,与对照相比,经过 EM 处理的水果中可以检测到更高的 H2O2 水平和 NOX 活性。然而,EM 增强的 H2O2 含量和 NOX 活性被二亚苯基氯化物部分削弱。这些结果表明,NOX 介导的 H2O2 是 EM 延迟采后枣果实衰老所必需的。因此,EM浸泡为我们提供了一种延缓果实采后衰老和提高营养品质的新方法。进一步的实验表明,与对照相比,经过 EM 处理的水果中可以检测到更高的 H2O2 水平和 NOX 活性。然而,EM 增强的 H2O2 含量和 NOX 活性被二亚苯基氯化物部分削弱。这些结果表明,NOX 介导的 H2O2 是 EM 延迟采后枣果实衰老所必需的。因此,EM浸泡为我们提供了一种延缓果实采后衰老和提高营养品质的新方法。进一步的实验表明,与对照相比,经过 EM 处理的水果中可以检测到更高的 H2O2 水平和 NOX 活性。然而,EM 增强的 H2O2 含量和 NOX 活性被二亚苯基氯化物部分削弱。这些结果表明,NOX 介导的 H2O2 是 EM 延迟采后枣果实衰老所必需的。因此,EM浸泡为我们提供了一种延缓果实采后衰老和提高营养品质的新方法。
更新日期:2020-08-01
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