Preeclampsia (PE) is a pregnancy-related syndrome and has become the leading cause of maternal and neonatal morbidity and mortality. LncRNA has been elucidated to play critical roles in the phenotype of trophoblast cells. However, the effect of AK002210 has not been reported. We aim to investigate the effect of AK002210 on the phenotype of trophoblast cells. Quantitative reverse transcription PCR was used to assess the gene expression. CCK-8 assay was used to evaluate the cell proliferation. Transwell assay was performed to detect the migration and invasion of trophoblast cells. Luciferase assay and rescue experiment were carried out to verify the interaction between miR-590-3p and AK002210 as well as NLR family apoptosis inhibitory protein (NAIP). The results revealed that AK002210 promoted the proliferation, migration and invasion of trophoblast cell while AK002210 knockdown inhibited that. Mechanically, we found that AK002210 was targeted by miR-590-3p. Moreover, miR-590-3p also directly targets NAIP which served as a ceRNA of AK002210. Rescue experiment showed that miR-590-3p reversed the effect of AK002210 which further confirmed their interaction. Moreover, AK002210 was proved to participated in the regulation of ERK/MMP-2 signal axis. In conclusion, we found that AK002210 knockdown may play a critical role in the progression of PE via miR-590-3p/NAIP and ERK/MMP signaling. It has potential to be a novel prognostic or therapeutic marker of PE.

中文翻译：

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AK002210通过调节miR-590 / NAIP信号轴促进滋养细胞的增殖，迁移和侵袭。

先兆子痫（PE）是一种与妊娠有关的综合征，已成为孕产妇和新生儿发病率和死亡率的主要原因。已经阐明了LncRNA在滋养细胞细胞表型中起关键作用。但是，尚未报道AK002210的作用。我们旨在研究AK002210对滋养细胞细胞表型的影响。定量逆转录PCR用于评估基因表达。使用CCK-8测定来评估细胞增殖。进行Transwell测定以检测滋养细胞的迁移和侵袭。进行荧光素酶测定和拯救实验以验证miR-590-3p与AK002210以及NLR家族凋亡抑制蛋白（NAIP）之间的相互作用。结果表明AK002210促进了增殖，滋养层细胞的迁移和侵袭，而AK002210的抑制则抑制了它。在机械上，我们发现AK002210被miR-590-3p靶向。而且，miR-590-3p还直接靶向用作AK002210的ceRNA的NAIP。救援实验表明，miR-590-3p逆转了AK002210的作用，进一步证实了它们的相互作用。此外，AK002210被证明参与ERK / MMP-2信号轴的调节。总之，我们发现AK002210敲低可能通过miR-590-3p / NAIP和ERK / MMP信号传导在PE进程中起关键作用。它有可能成为PE的新的预后或治疗标志。救援实验表明，miR-590-3p逆转了AK002210的作用，进一步证实了它们的相互作用。此外，AK002210被证明参与ERK / MMP-2信号轴的调节。总之，我们发现AK002210敲低可能通过miR-590-3p / NAIP和ERK / MMP信号传导在PE进程中起关键作用。它有可能成为PE的新的预后或治疗标志。救援实验表明，miR-590-3p逆转了AK002210的作用，进一步证实了它们的相互作用。此外，AK002210被证明参与ERK / MMP-2信号轴的调节。总之，我们发现AK002210敲低可能通过miR-590-3p / NAIP和ERK / MMP信号传导在PE进程中起关键作用。它有可能成为PE的新的预后或治疗标志。