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Magnetic particles for integrated nucleic acid purification, amplification and detection without pipetting.
Trends in Analytical Chemistry ( IF 11.8 ) Pub Date : 2020-05-06 , DOI: 10.1016/j.trac.2020.115912 Yanju Chen 1 , Yang Liu 2 , Ya Shi 2 , Jianfeng Ping 1 , Jian Wu 1, 3 , Huan Chen 2
Trends in Analytical Chemistry ( IF 11.8 ) Pub Date : 2020-05-06 , DOI: 10.1016/j.trac.2020.115912 Yanju Chen 1 , Yang Liu 2 , Ya Shi 2 , Jianfeng Ping 1 , Jian Wu 1, 3 , Huan Chen 2
Affiliation
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Nucleic acid amplification based detection plays an important role in food safety, environmental monitoring and clinical diagnosis. However, traditional nucleic acid detection process involves transferring liquid from one tube to another by pipetting It requires trained persons, equipped labs and consumes lots of time. The ideal nucleic acid detection is integrated, closed, simplified and automated. Magnetic particles actuated by magnetic fields can efficiently adsorb nucleic acids and promote integrated nucleic acid assays without pipetting driven by pumps and centrifuges. We will comprehensively review magnetic particles assisted integrated system for nucleic acid detection and hope it can inspire further related study.
中文翻译：
用于集成核酸纯化、扩增和检测的磁性颗粒，无需移液。
基于核酸扩增的检测在食品安全、环境监测和临床诊断中发挥着重要作用。然而，传统的核酸检测过程需要通过移液器将液体从一根试管转移到另一根试管，需要经过培训的人员、配备齐全的实验室并消耗大量时间。理想的核酸检测是集成的、封闭的、简化的、自动化的。由磁场驱动的磁性颗粒可以有效地吸附核酸并促进整合核酸测定，而无需泵和离心机驱动的移液。我们将对磁性粒子辅助核酸检测集成系统进行全面综述，希望对进一步的相关研究有所启发。
更新日期：2020-05-06
中文翻译：
用于集成核酸纯化、扩增和检测的磁性颗粒，无需移液。
基于核酸扩增的检测在食品安全、环境监测和临床诊断中发挥着重要作用。然而，传统的核酸检测过程需要通过移液器将液体从一根试管转移到另一根试管，需要经过培训的人员、配备齐全的实验室并消耗大量时间。理想的核酸检测是集成的、封闭的、简化的、自动化的。由磁场驱动的磁性颗粒可以有效地吸附核酸并促进整合核酸测定，而无需泵和离心机驱动的移液。我们将对磁性粒子辅助核酸检测集成系统进行全面综述，希望对进一步的相关研究有所启发。
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