Acidification of animal slurry is recommended to reduce NH₃ emissions, but little is known about how such slurry influences soil microorganisms. Here we test the impact of acidified pig slurry, untreated slurry, and mineral N–S fertiliser on soil dehydrogenase activity (DHA), microbial community composition (PLFA), soil pH, and SO₄-S, NO₃-N, and NH₄-N content in the 0–20 cm layer in winter wheat (Triticum aestivum L.) plots in Estonia in a field experiment conducted in 2017 and 2018. The slurry was acidified with H₂SO₄ (96%) to reach the target pH of 6.0 (to imitate the in-storage acidification system). The soil was sampled twice each year: 2 weeks after slurry spreading and at winter wheat harvest. In both years, the spreading of acidified slurry significantly increased the SO₄-S content in the soil. The level of SO₄-S was remarkably higher 2 weeks after slurry spreading, then decreased towards harvest, but remained significantly higher compared to other treatments. Compared to the untreated slurry, the use of acidified slurry had no significant (p < 0.05) effect on soil pH or NO₃-N, and NH₄-N content, nor soil DHA and microbial community composition. Only the relative abundance of Actinobacteria was significantly different between treatments, which is likely due do the added nitrogen in the NO₃-N.

建议对动物粪便进行酸化处理以减少NH ₃排放，但对于这种粪便如何影响土壤微生物的了解很少。在这里，我们测试了酸化的猪粪，未处理的粪浆和矿物N–S肥料对土壤脱氢酶活性（DHA），微生物群落组成（PLFA），土壤pH以及SO ₄ -S，NO ₃ -N和NH的影响在2017年和2018年进行的田间试验中，爱沙尼亚冬小麦（Triticum aestivum L.）地块0–20 cm层中的₄ -N含量。将浆液用H ₂ SO ₄酸化（96％）达到6.0的目标pH（模仿存储内酸化系统）。每年两次对土壤取样：泥浆铺开后两周和冬小麦收获时。在这两年中，酸化浆液的扩散显着增加了土壤中SO ₄ -S的含量。浆液撒播2周后，SO ₄ -S含量显着升高，然后朝收获期下降，但与其他处理相比仍显着较高。与未处理的泥浆相比，使用酸化的泥浆对土壤pH或NO ₃ -N和NH ₄ -N含量无显着（p <0.05）影响，对土壤DHA和微生物群落组成也无明显影响。仅放线菌的相对丰度两次处理之间存在显着差异，这很可能是因为NO ₃ -N中添加了氮。