Interleukins (ILs) regulate cell surface antigens known as activation markers, which have distinct functional roles. However, the regulation of major histocompatibility complex (MHC) class I, MHC class II, and related genes by cytokines in chickens is not well understood. In the present study, we evaluated the influence of certain recently discovered chicken interleukins-i.e., IL-11, IL-12B, IL-17A, IL-17B, IL-26, and IL-34-on the expression and regulation of genes related to MHC class I, MHC class II, and the associated proteins in an HD11 chicken macrophage cell line. We used quantitative reverse transcription polymerase chain reaction (qRT-PCR), immunocytochemical, and flow cytometric analyses to assess dose- and time-dependent expression in the HD11 cell line and found that the ILs induced MHC class I, MHC class II, and associated protein. As NF-κB is actively involved in cell activation and is constitutively activated in many immune cells, we also determined whether NF-κB regulates MHC class I, MHC class II, and related gene expression in the HD11 cell line. The NF-κB inhibitor sulfasalazine (Sz) dose-dependently inhibited MHC class I and MHC class II in the HD11 cell line. Sz also downregulated the expression of MHC class I, MHC class II, and the associated proteins in the IL-induced HD11 cell line. The expression of MHC class I, MHC class II, and associated genes was accompanied by the Sz-sensitive degradation of the p65 (RelA) and p50 subunits of NF-κB and IκBα. Our results indicate that the different effects of each IL on the expression of genes related to MHC class I, MHC class II, and the associated proteins are involved with the regulation of the dose and duration of antigenic peptide presentation and, thus, also influence Th1, Th2, and Th17 production.

中文翻译：

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鸡 HD11 细胞中 MHC I 类和 MHC II 类基因表达的白介素依赖性调节。

白细胞介素 (IL) 调节称为激活标记物的细胞表面抗原，具有不同的功能作用。然而，细胞因子对鸡中主要组织相容性复合体（MHC）I类、MHC II类和相关基因的调节尚不清楚。在本研究中，我们评估了某些最近发现的鸡白介素——即 IL-11、IL-12B、IL-17A、IL-17B、IL-26 和 IL-34——对基因表达和调控的影响与 HD11 鸡巨噬细胞系中的 MHC I 类、MHC II 类和相关蛋白相关。我们使用定量逆转录聚合酶链反应 (qRT-PCR)、免疫细胞化学和流式细胞术分析来评估 HD11 细胞系中的剂量和时间依赖性表达，发现 ILs 诱导 MHC I 类、MHC II 类和相关的蛋白质。由于 NF-κB 积极参与细胞活化并在许多免疫细胞中组成型激活，我们还确定了 NF-κB 是否调节 MHC I 类、MHC II 类和 HD11 细胞系中的相关基因表达。NF-κB 抑制剂柳氮磺胺吡啶 (Sz) 剂量依赖性地抑制 HD11 细胞系中的 MHC I 类和 MHC II 类。Sz 还下调了 IL 诱导的 HD11 细胞系中 MHC I 类、MHC II 类和相关蛋白质的表达。MHC I 类、MHC II 类和相关基因的表达伴随着 NF-κB 和 IκBα 的 p65 (RelA) 和 p50 亚基的 Sz 敏感性降解。我们的结果表明，每种 IL 对 MHC I 类、MHC II 类、