The suppressor of cytokine signaling 3 (SOCS3), as a negative regulator in inferferon (IFN) signaling pathways in mammals, has a vital role in immune systems. However, studies on the function of SOCS3 in lower vertebrates are limited. In this study, we identified SOCS3a and fish-specific SOCS3b gene in miiuy croaker. Sequence analysis results showed that SOCS3a and SOCS3b were evolutionarily conservative in fish. Expression analysis indicated that miiuy croaker SOCS3a and SOCS3b (mmSOCS3a and mmSOCS3b) were expressed in all of the tested miiuy croaker tissues, thus revealing the potential ability to perceive poly (I:C) stimulation. Further functional experiments showed that mmSOCS3a and mmSOCS3b could inhibit the IFNγ- and IFNα-induced ISRE reporter activation, respectively. Accordingly, the investigation of mmSOCS3a and mmSOCS3b can provide insights into fish SOCS3 and a basis for future research on the SOCS family of fish immune systems.

中文翻译：

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鲥鱼 (Miichthys miiuy) 中 SOCS3a 和 SOCS3b 的分子进化和功能表征。

细胞因子信号抑制因子 3 (SOCS3) 作为哺乳动物干扰素 (IFN) 信号通路的负调节因子，在免疫系统中具有重要作用。然而，关于SOCS3在低等脊椎动物中的功能的研究是有限的。在这项研究中，我们在 miiuy croaker 中鉴定了 SOCS3a 和鱼类特异性 SOCS3b 基因。序列分析结果表明SOCS3a和SOCS3b在鱼类中进化保守。表达分析表明 miiuy croaker SOCS3a 和 SOCS3b（mmSOCS3a 和 mmSOCS3b）在所有测试的 miiuy croaker 组织中均有表达，从而揭示了感知聚 (I:C) 刺激的潜在能力。进一步的功能实验表明，mmSOCS3a 和 mmSOCS3b 可以分别抑制 IFNγ 和 IFNα 诱导的 ISRE 报告基因激活。因此，