Vibrio harveyi is regarded as serious pathogen for marine fishes. To evaluate the physiological responses of spotted sea bass (Lateolabrax maculatus) after V. harveyi infection, four biochemical biomarkers including alanine amino transferase (ALT), albumin (ALB), total protein (TP) and glucose (GLU) were measured in serum. Our results showed that V. harveyi infection significantly influenced the concentration of ALT, ALB and GLU. Additionally, five interleukin-17 (IL-17) and five IL-17 receptors (IL-17R) genes were identified in spotted sea bass and their gene structures were characterized. Furthermore, the expression patterns of IL-17 and IL-17R genes were determined by qPCR in liver, intestine, spleen and head kidney after V. harveyi infection. All IL-17 and IL-17R genes exhibited time- and tissue-dependent expressions. Several tested genes were dramatically induced by V. harveyi treatment, particularly IL-17A/F1 in liver and head kidney, IL-17A/F2 in head kidney, IL-17RC in spleen with more than 10-fold increases, which suggested their potential essential roles against bacterial infection.

中文翻译：

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哈维伊弧菌感染对斑点鲈鱼血清生化参数和白细胞介素17（IL-17）/白细胞介素17受体（IL-17R）基因表达谱的影响。

哈维弧菌被认为是海洋鱼类的严重病原体。为了评估斑鲈（Lateolabrax maculatus）在 V. harveyi 感染后的生理反应，在血清中测量了四种生化生物标志物，包括丙氨酸氨基转移酶 (ALT)、白蛋白 (ALB)、总蛋白 (TP) 和葡萄糖 (GLU)。我们的结果表明，哈维伊弧菌感染显着影响了 ALT、ALB 和 GLU 的浓度。此外，在斑点鲈鱼中鉴定了五个白细胞介素-17 (IL-17) 和五个 IL-17 受体 (IL-17R) 基因，并对它们的基因结构进行了表征。此外，IL-17 和 IL-17R 基因的表达模式通过 qPCR 在哈维伊弧菌感染后的肝、肠、脾和头肾中确定。所有 IL-17 和 IL-17R 基因都表现出时间和组织依赖性表达。