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Genetically encoded protein labeling and crosslinking in living Pseudomonas aeruginosa.
Bioorganic & Medicinal Chemistry ( IF 3.3 ) Pub Date : 2020-05-06 , DOI: 10.1016/j.bmc.2020.115545
Huangtao Zheng 1 , Shixian Lin 2 , Peng R Chen 3
Affiliation  

Pseudomonas aeruginosa (PA) is a major human pathogen for hospital-acquired infections. We report the genetic code expansion of this opportunistic pathogen by using the pyrrolysyl-tRNA synthetase-tRNA system, which enabled the genetic and site-specific incorporation of unnatural amino acids bearing bioorthogonal handles or photo-affinity groups into proteins in PA. This strategy allowed us to conduct bioorthogonal labeling and imaging of flagella, as well as site-specific photo-affinity capturing of interactions between a Type III secretion effector and its chaperone inside living bacteria.



中文翻译:

遗传编码的蛋白质标记和活铜绿假单胞菌中的交联。

铜绿假单胞菌(PA)是医院获得性感染的主要人类病原体。我们通过使用吡咯基-tRNA合成酶-tRNA系统报告了这种机会病原体的遗传密码扩展,该系统使携带生物正交柄或光亲和力基团的非天然氨基酸的遗传和位点特异性整合成为PA中的蛋白质。这种策略使我们能够进行鞭毛的生物正交标记和成像,以及在活细菌中对III型分泌效应物及其伴侣进行相互作用的特定位置的光亲和性捕获。

更新日期:2020-05-06
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