Emerging evidence shows that Rab11 recycling endosomes (REs Rab11) are essential for several neuronal processes, including the proper functioning of growth cones, synapse architecture regulation and neuronal migration. However, several aspects of REs Rab11 remain unclear, such as its sub-cellular distribution across neuronal development, contribution to dendritic tree organization and its consequences in memory formation. In this work we show a spatio-temporal correlation between the endogenous localization of REs Rab11 and developmental stage of neurons. Furthermore, Rab11-suppressed neurons showed an increase on dendritic branching (without altering total dendritic length) and misdistribution of dendritic proteins in cultured neurons. In addition, suppression of Rab11 in adult rat brains in vivo (by expressing shRab11 through lentiviral infection), showed a decrease on both the sensitivity to induce long-term potentiation and hippocampal-dependent memory acquisition. Taken together, our results suggest that REs Rab11 expression is required for a proper dendritic architecture and branching, controlling key aspects of synaptic plasticity and spatial memory formation.

中文翻译：

---

Rab11的减少会阻止正确的树突乔化，突触可塑性和空间记忆的形成。

新兴证据表明，Rab11回收内体（RE Rab11）对于几种神经元过程至关重要，包括生长锥的正常功能，突触结构调节和神经元迁移。然而，RE Rab11的几个方面仍不清楚，例如其在神经元发育中的亚细胞分布，对树突树组织的贡献及其在记忆形成中的后果。在这项工作中，我们显示了REs Rab11的内源性定位与神经元发育阶段之间的时空相关性。此外，Rab11抑制的神经元显示树突分支的增加（不改变总树突长度）和在培养的神经元中树突蛋白的分布不均。此外，体内抑制成年大鼠大脑中Rab11的表达（通过慢病毒感染表达shRab11）显示出诱导长期增强的敏感性和海马依赖性记忆习性均降低。综上所述，我们的结果表明，REs Rab11表达对于适当的树突结构和分支，控制突触可塑性和空间记忆形成的关键方面是必需的。