Skin lesions are normal to all species, regardless of gender or age. The skin, the largest organ of the body, has function as a primary barrier to the chemical, physical and biological aggressions of the environment. In animals, these lesions may be due to fights and/or predations, also as in humans, there is a very common cause of dermal lesions that are caused by burns and carcinomas. Looking for new techniques of tissue bioengineering, studies have been shown promising results for formulations of acellular biological scaffolds from tissue decellularization for the reconstitution of these lesions. The decellularization has its proof by a varied range of tests such as scanning electron microscopy and residual genomic DNA tests. Subsequently the tissue can go through the process of recellularization using cells of interest, even the animal that will receive this tissue, reducing the risks of rejection and improving the response to tissue transplantation. Thus, this manuscript aimed at the decellularization of the tissue with the use of chemical and physical means followed by sterilization and the establishment of a protocol for the recellularization of a decellularized scaffold from the Wistar rat dermis using murine fibroblasts and mesenchymal stem cells from canine adipose tissue for 7 days. After efficacy tests, the tissue recellularization were confirmed by immunofluorescence assays and scanning electron microscopy where the adherence of the cells in the biological scaffold was observed.

中文翻译：

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Wistar大鼠真皮再细胞化。

不论性别或年龄，皮肤病变对所有物种都是正常的。皮肤是人体最大的器官，对环境的化学，物理和生物侵害具有主要的屏障作用。在动物中，这些损伤可能是由于打架和/或掠食造成的，就像在人类中一样，皮肤灼伤和癌变是引起皮肤损伤的非常普遍的原因。在寻找组织生物工程学的新技术时，研究表明，从组织脱细胞中重组无细胞生物支架以重建这些病灶的结果令人鼓舞。脱细胞化通过各种测试方法来证明，例如扫描电子显微镜和残留基因组DNA测试。随后，组织可以使用感兴趣的细胞经历重新细胞化的过程，即使是将接受这种组织的动物，也可以减少排斥的风险并改善对组织移植的反应。因此，该手稿旨在通过化学和物理手段对组织进行脱细胞，然后进行灭菌，并建立了使用鼠成纤维细胞和犬脂肪间充质干细胞对来自Wistar大鼠真皮的脱细胞支架进行脱细胞的方法组织7天。在功效测试之后，通过免疫荧光测定法和扫描电子显微镜确认了组织的重新细胞化，其中观察到了细胞在生物支架中的粘附。该手稿旨在通过化学和物理手段对组织进行脱细胞，然后进行灭菌，并建立了使用鼠成纤维细胞和来自犬脂肪组织的间充质干细胞对来自Wistar大鼠真皮的脱细胞支架进行细胞脱细胞化的方案。 7天。在功效测试后，通过免疫荧光测定法和扫描电子显微镜确认了组织再细胞化，其中观察到了细胞在生物支架中的粘附。该手稿旨在通过化学和物理手段对组织进行脱细胞，然后进行灭菌，并建立了使用鼠成纤维细胞和犬脂肪组织的间充质干细胞对来自Wistar大鼠真皮的脱细胞支架进行去细胞化的方案。 7天。在功效测试后，通过免疫荧光测定法和扫描电子显微镜确认了组织再细胞化，其中观察到了细胞在生物支架中的粘附。