Abstract This study assessed ovarian dynamics in picarel and examined how it can be implemented in routine calculations of batch fecundity and spawning interval. Samples of picarel were collected in Saronikos Gulf, Greece, and ovaries were examined through both histological and whole mount procedures. Three different and independent approaches were applied to assess ovarian modality: (a) the multimodal analysis in oocyte size frequency distributions (OSFD), (b) the histological grouping of oocytes to different cohorts and (c) the ratio of the total number of secondary growth oocytes to the oocytes of the advanced mode. The output from all three methods was in good agreement to each other, suggesting that picarel contains in average 3 to 5 oocyte cohorts throughout the spawning period. Further study of OSFD revealed that the advanced oocyte mode (AM) was clearly distinct since the onset of the spawning season in February and it either partially overlapped with or was separated from smaller oocytes. The gravimetric estimate of relative fecundity of the advanced mode (RFAM) in hydrated females equaled to 336 oocytes g−1 (± standard error16.23) and was comparable to the RFAM from other stages, with and without hiatus in the OSFD. These results show that batch fecundity in picarel can be estimated even in non-hydrated females. Considering the temperature dependent duration of posyovulatory follicle (POF) degeneration, the prevalence of recent spawning markers and their co-occurrence with imminent spawning markers, we conclude that spawning interval in picarel at peak spawning should be between 2 and 4 days.

中文翻译：

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picarel (Spicara smaris, L., Sparidae) 的卵巢动态及其对批次繁殖力和产卵间隔估计的影响

摘要 本研究评估了 picarel 中的卵巢动态，并检查了如何在批次繁殖力和产卵间隔的常规计算中实施它。在希腊的萨罗尼科斯湾收集了 picarel 样品，并通过组织学和整体安装程序检查了卵巢。应用三种不同且独立的方法来评估卵巢形态：(a) 卵母细胞大小频率分布 (OSFD) 的多模态分析，(b) 卵母细胞在不同队列中的组织学分组，以及 (c) 次生卵母细胞总数的比率生长卵母细胞到高级模式的卵母细胞。所有三种方法的输出都非常一致，表明在整个产卵期间，picarel 平均包含 3 到 5 个卵母细胞群。OSFD 的进一步研究表明，高级卵母细胞模式 (AM) 自 2 月产卵季节开始就明显不同，它与较小的卵母细胞部分重叠或分离。水合雌性中高级模式 (RFAM) 的相对繁殖力的重量估计值等于 336 个卵母细胞 g-1（± 标准误差 16.23），并且与来自其他阶段的 RFAM 相当，无论 OSFD 有无中断。这些结果表明，即使在未水合的雌性中，也可以估计 picarel 的批次繁殖力。考虑到排卵后卵泡 (POF) 变性的温度依赖性持续时间、最近产卵标记的流行及其与即将产卵标记的共同出现，我们得出结论，产卵高峰期 picarel 的产卵间隔应在 2 到 4 天之间。