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Isolation and identification of lactic acid bacteria in fresh plants and in silage from Opuntia and their effects on the fermentation and aerobic stability of silage
The Journal of Agricultural Science ( IF 1.7 ) Pub Date : 2020-03-25 , DOI: 10.1017/s0021859620000143
G. A. Pereira , E. M. Santos , G. G. L. Araújo , J. S. Oliveira , R. M. A. Pinho , A. de M. Zanine , A. F. N. Souza , A. J. S. Macedo , J. M. C. Neto , T. V. C. Nascimento

The current study aimed to select the strains of lactic acid bacteria (LAB) isolated from forage cactus plants and silage and assess their effects on silage fermentation and aerobic stability. Forty wild isolates from plant and cactus silage, classified as LAB, were evaluated for metabolite production and identified by 16S ribosomal DNA sequencing. These wild isolates were identified asLactobacillus plantarum,Weissella cibaria,Weissella confusaandWeissella paramesenteroidesand the LAB populations differed among the silage. The use of microbial inoculants did not influence gas or effluent losses in forage cactus silage. The silage inoculated with the microbial strain GP15 showed the highest number of LAB populations. The amounts of water-soluble carbohydrates (WSC) and ammonia nitrogen differed among the silage. The silage inoculated with the GP1 strain presented the highest WSC. Populations of enterobacteria and yeasts and moulds were below the minimum detection limit (<2.0 log cfu/g silage) in all the silage studied. The predominant action of inoculants was to maximize dry matter recovery of the silage, which could be the criterion adopted to select the strains of LAB for use as inoculants inOpuntiasilage.

中文翻译:

仙人掌新鲜植物和青贮饲料中乳酸菌的分离鉴定及其对青贮饲料发酵和好氧稳定性的影响

目前的研究旨在选择从饲料仙人掌植物和青贮饲料中分离的乳酸菌 (LAB) 菌株,并评估它们对青贮饲料发酵和有氧稳定性的影响。对来自植物和仙人掌青贮饲料的 40 个野生分离物(归类为 LAB)进行了代谢物生产评估,并通过 16S 核糖体 DNA 测序进行鉴定。这些野生分离株被鉴定为植物乳杆菌,雪松,白毛白蛉副肠系膜魏氏菌青贮饲料中的 LAB 种群不同。微生物接种剂的使用不影响饲料仙人掌青贮中的气体或流出物损失。接种微生物菌株 GP15 的青贮饲料显示出最高数量的 LAB 种群。青贮饲料中水溶性碳水化合物(WSC)和氨氮的含量不同。接种GP1菌株的青贮饲料具有最高的WSC。在所有研究的青贮饲料中,肠杆菌、酵母菌和霉菌的数量均低于最低检测限(<2.0 log cfu/g 青贮饲料)。接种剂的主要作用是最大限度地提高青贮饲料的干物质回收率,这可以作为选择乳酸菌菌株用作接种剂的标准。仙人掌青贮饲料。
更新日期:2020-03-25
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