Influence of Culture Conditions on Poly‐3‐Hydroxybutyrate Production by a Newly Isolated Bacillus cereus Y23,Clean - Soil Air Water

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Influence of Culture Conditions on Poly‐3‐Hydroxybutyrate Production by a Newly Isolated Bacillus cereus Y23
Clean - Soil Air Water ( IF 1.5 ) Pub Date : 2020-05-24 , DOI: 10.1002/clen.201900415
Oxana Yashchuk _{1,

2} , Élida B Hermida _{1,

2}

Affiliation

Polyhydroxyalkanoates (PHAs), environmental friendly polyesters, can be produced by a wide range of microorganisms in nutrient‐limiting conditions. In the present study a new bacterial strain, able to produce high quantities of poly‐3‐hydroxybutyrate (PHB), is isolated from contaminated soil. The isolate is characterized and identified as Bacillus cereus Y23, based on morphological, physiological characteristics, and 16S ribosomal RNA gene sequences. PHB production is evaluated using different carbon and nitrogen sources and nutritional and culture conditions are optimized. The highest PHB production of 5.12 g L⁻¹ was obtained in growth medium containing fructose and ammonium sulfate at a C/N ratio of 30, 0.3% yeast extract, 4.5 g L⁻¹ phosphate solution, pH 7, inoculum size of 0.3%, and agitation rate of 150 rpm after 48 h of incubation at 32 °C. The PHB production decrement can be attributed to the intracellular utilization of the polymer during the stationary phase of the cellular growth and to the sporulation process. The presence of the main functional groups of PHB polymer is verified by Fourier‐transform infrared analysis. At optimum conditions, B. cereus Y23 turned out to be a good candidate for industrial production of PHB since it accumulates up to 67.9% of its dry weight.

中文翻译：

培养条件对新分离的蜡状芽孢杆菌Y23产生聚3-羟基丁酸的影响

聚羟基链烷酸酯（PHA）是环境友好的聚酯，可以在营养限制条件下由多种微生物生产。在本研究中，从受污染的土壤中分离出了一种新型细菌菌株，该菌株能够产生大量的聚3-羟基丁酸酯（PHB）。根据形态，生理特征和16S核糖体RNA基因序列，将分离物鉴定为蜡样芽孢杆菌Y23。使用不同的碳和氮源评估PHB的生产，并优化营养和培养条件。最高PHB产量5.12克L- ^-1在含有果糖和硫酸铵在30 C / N比，0.3％酵母提取物，4.5克L-生长培养基得到^-1磷酸盐溶液，pH 7，接种量为0.3％，在32°C孵育48小时后的搅拌速度为150 rpm。PHB产生的减少可归因于在细胞生长的稳定阶段聚合物在细胞内的利用以及孢子形成过程。PHB聚合物的主要官能团的存在通过傅里叶变换红外分析得以证实。在最佳条件下，蜡状芽孢杆菌Y23被证明是PHB工业生产的良好候选者，因为它积累的干重高达67.9％。

更新日期：2020-05-24

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