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Seasonal variation in testicular blood flow dynamics and their relation to systemic and testicular oxidant/antioxidant biomarkers and androgens in rams.
Reproduction in Domestic Animals ( IF 1.6 ) Pub Date : 2020-05-06 , DOI: 10.1111/rda.13696
Mohamed G Hedia 1 , Mohamed S El-Belely 1 , Sayed T Ismail 1 , Amal M Abo El-Maaty 2
Affiliation  

The environmental temperature increased during summer and decreased during winter to the limits that might negatively affect animal and human reproduction. The responses of Egyptian rams to either hot or cold climatic conditions were studied in six mature rams subjected to weekly testicular Doppler ultrasonographic examination, blood sampling, seminal plasma collection and semen evaluation. The maximum environmental temperature and the relative humidity were used to classify the climatic condition according to the heat stress equation of sheep into hot months where temperature–humidity index (THI) was >26 (31.67 ± 0.54), and cold months where THI was <22 (18.39 ± 0.41). Testosterone, estradiol, superoxide dismutase (SOD), glutathione peroxidase (GPX) and lipid peroxide product (malondialdehyde, MDA) were measured in both blood and seminal plasma, while catalase (CAT) and reduced glutathione (GSH) were measured in blood and seminal plasma, respectively. Results revealed that, during the hot months, rams displayed significantly decreased testicular blood flow, increased seminal plasma MDA, decreased seminal plasma (SOD, GPx and GSH) and blood CAT antioxidant enzymes. The present study evidenced two novel findings: (a) the marked decrease in testicular blood flow volume, that is remarkable increase in both resistive index (RI) and pulsatility index (PI) values, during hot months could be negatively affected both seminal plasma enzymatic activities and seminal attributes, and (b) the SOD and GPx activities in seminal plasma of such animals were suitable predictive markers for seminal attribute evaluation.

中文翻译:

公羊睾丸血流动力学的季节性变化及其与全身和睾丸氧化剂/抗氧化剂生物标志物和雄激素的关系。

环境温度在夏季升高,在冬季降低到可能对动物和人类繁殖产生不利影响的极限。在每周进行睾丸多普勒超声检查,血液采样,精浆收集和精液评估的六个成熟公羊中,研究了埃及公羊对炎热或寒冷气候条件的反应。根据绵羊的热应激方程,使用最高环境温度和相对湿度对气候条件进行分类,将其分为温度-湿度指数(THI)> 26(31.67±0.54)的炎热月份和THI << 22(18.39±0.41)。睾丸激素,雌二醇,超氧化物歧化酶(SOD),谷胱甘肽过氧化物酶(GPX)和脂质过氧化物产品(丙二醛,分别在血液和精浆中测量MDA),同时在血液和精浆中测量过氧化氢酶(CAT)和还原型谷胱甘肽(GSH)。结果显示,在炎热的月份中,公羊的睾丸血流量显着下降,精浆MDA升高,精浆(SOD,GPx和GSH)和血液CAT抗氧化酶降低。本研究证明了两个新发现:(a)睾丸血流量显着减少,即在炎热月份,阻力指数(RI)和脉搏指数(PI)值均显着增加,这可能会对精浆血浆酶产生不利影响(b)这些动物精液中的SOD和GPx活性是评估精液属性的合适预测指标。分别在血液和精浆中测定过氧化氢酶(CAT)和还原型谷胱甘肽(GSH)。结果显示,在炎热的月份中,公羊的睾丸血流量显着下降,精浆MDA升高,精浆(SOD,GPx和GSH)和血液CAT抗氧化酶降低。本研究证明了两个新发现:(a)睾丸血流量显着减少,即在炎热月份,阻力指数(RI)和脉搏指数(PI)值均显着增加,这可能会对精浆血浆酶产生不利影响(b)这些动物精液中的SOD和GPx活性是评估精液属性的合适预测指标。分别在血液和精浆中测定过氧化氢酶(CAT)和还原型谷胱甘肽(GSH)。结果显示,在炎热的月份中,公羊的睾丸血流量显着下降,精浆MDA升高,精浆(SOD,GPx和GSH)和血液CAT抗氧化酶降低。本研究证明了两个新发现:(a)睾丸血流量显着减少,即在炎热月份,阻力指数(RI)和脉搏指数(PI)值均显着增加,这可能会对精浆血浆酶产生不利影响活性和精液属性,以及(b)这些动物精浆中的SOD和GPx活性是评估精液属性的合适预测指标。结果显示,在炎热的月份中,公羊的睾丸血流量显着下降,精浆MDA升高,精浆(SOD,GPx和GSH)和血液CAT抗氧化酶降低。本研究证明了两个新发现:(a)睾丸血流量显着减少,即在炎热月份,阻力指数(RI)和脉搏指数(PI)值均显着增加,这可能会对精浆血浆酶产生不利影响(b)这些动物精液中的SOD和GPx活性是评估精液属性的合适预测指标。结果显示,在炎热的月份中,公羊的睾丸血流量显着下降,精浆MDA升高,精浆(SOD,GPx和GSH)和血液CAT抗氧化酶降低。本研究证明了两个新发现:(a)睾丸血流量显着减少,即在炎热月份,阻力指数(RI)和脉搏指数(PI)值均显着增加,这可能会对精浆血浆酶产生不利影响(b)这些动物精液中的SOD和GPx活性是评估精液属性的合适预测指标。GPx和GSH)和血液CAT抗氧化酶。本研究证明了两个新发现:(a)睾丸血流量显着减少,即在炎热月份,阻力指数(RI)和脉搏指数(PI)值均显着增加,这可能会对精浆血浆酶产生不利影响(b)这些动物精液中的SOD和GPx活性是评估精液属性的合适预测指标。GPx和GSH)和血液CAT抗氧化酶。本研究证明了两个新发现:(a)睾丸血流量显着减少,即在炎热月份,阻力指数(RI)和脉搏指数(PI)值均显着增加,这可能会对精浆血浆酶产生不利影响(b)这些动物精液中的SOD和GPx活性是评估精液属性的合适预测指标。
更新日期:2020-07-13
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