Nitrogen (N) is a major factor for plant development and productivity. However, the application of nitrogenous fertilizers generates environmental and economic problems. To cope with the increasing global food demand, the development of rice varieties with high nitrogen use efficiency (NUE) is indispensable for reducing environmental issues and achieving sustainable agriculture. Here, we report that the concomitant activation of the rice (Oryza sativa ) Ammonium transporter 1;2 (OsAMT1;2 ) and Glutamate synthetase 1 (OsGOGAT1) genes leads to increased tolerance to nitrogen limitation and to better ammonium uptake and N remobilization at the whole plant level. We show that the double activation of OsAMT1;2 and OsGOGAT1 increases plant performance in agriculture, providing better N grain filling without yield penalty under paddy field conditions, as well as better grain yield and N content when plants are grown under N llimitations in field conditions. Combining OsAMT1;2 and OsGOGAT1 activation provides a good breeding strategy for improving plant growth, nitrogen use efficiency and grain productivity, especially under nitrogen limitation, through the enhancement of both nitrogen uptake and assimilation.

中文翻译：

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在氮限制下，水稻中OsAMT1; 2和OsGOGAT1的同时激活导致氮的利用效率提高。

氮是植物生长和生产力的主要因素。然而，氮肥的施用产生了环境和经济问题。为了应对不断增长的全球粮食需求，开发具有高氮利用效率（NUE）的水稻品种对于减少环境问题和实现可持续农业是必不可少的。在这里，我们报道水稻（Oryza sativa）铵转运蛋白1; 2（OsAMT1; 2）和谷氨酸合成酶1（OsGOGAT1）基因的同时活化导致对氮限制的耐受性提高，并提高了铵吸收和氮的转运。全厂水平。我们证明了OsAMT1; 2的双重激活和OsGOGAT1增加农业植物性能，提供更好的Ñ灌浆而不水田条件下产量损失，以及更好的产量和N含量当植物在田间条件下Ñllimitations生长。组合OsAMT1; 2和OsGOGAT1活化提供了用于改善植物生长，氮利用效率和粮食生产，特别是在氮限制，通过两个氮摄取和同化的增强良好的育种策略。