Participation of TRPV1 in the activity of the GnRH system in male rats.,European Journal of Neuroscience

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Participation of TRPV1 in the activity of the GnRH system in male rats.
European Journal of Neuroscience ( IF 2.7 ) Pub Date : 2020-05-20 , DOI: 10.1111/ejn.14770
Pablo Nicolás Surkin _{1,

2} , Ganna Dmytrenko ₃ , Noelia Paula Di Giorgio ₄ , Marianne Bizzozzero ₄ , Andrea De Laurentiis ₃ , Javier Fernández-Solari _{1,

2}

Affiliation

GnRH neuron activity is under the influence of multiple stimuli, including those coming from the endocannabinoid and the immune systems. Since it has been previously suggested that some of the main elements controlling the GnRH pulse generator possess the TRPV1 receptor, the aim of the present study was to evaluate the participation of the hypothalamic TRPV1, through its pharmacological blockade, in the activity of the hypothalamic‐pituitary‐testicular axis in male rats under basal or acute inflammatory conditions. Our hypothesis was based on the idea that the hypothalamic TRPV1 participates in the synthesis of the main neuromodulatory signals controlling GnRH, and therefore the reproductive axis. Our results showed that the hypothalamic TRPV1 blockade induced pro‐inflammatory effects by increasing Tnfα and Il‐1β mRNA hypothalamic levels and inhibited the reproductive axis by affecting Gnrh, Kiss1 and Rfrp3 mRNA levels and decreasing plasma levels of luteinizing hormone and testosterone under basal conditions, without significant additive effects in rats exposed to systemic LPS. Altogether, these results suggest that the hypothalamic TRPV1 receptor participates in the regulation of the GnRH system, probably by modulating immune‐dependent mechanisms.

中文翻译：

TRPV1 参与雄性大鼠 GnRH 系统的活动。

GnRH 神经元活动受到多种刺激的影响，包括来自内源性大麻素和免疫系统的刺激。由于之前已经提出控制 GnRH 脉冲发生器的一些主要元素具有 TRPV1 受体，本研究的目的是评估下丘脑 TRPV1 通过其药理学阻断参与下丘脑的活性。雄性大鼠在基础或急性炎症条件下的垂体-睾丸轴。我们的假设是基于下丘脑 TRPV1 参与控制 GnRH 的主要神经调节信号的合成，因此参与了生殖轴的合成。我们的结果表明，下丘脑 TRPV1 阻断通过增加Tnfα和Il-1β mRNA 下丘脑水平并通过影响Gnrh、Kiss1和Rfrp3 mRNA 水平以及在基础条件下降低促黄体激素和睾酮的血浆水平来抑制生殖轴，在暴露于全身 LPS 的大鼠中没有显着的累加效应。总之，这些结果表明下丘脑 TRPV1 受体可能通过调节免疫依赖性机制参与 GnRH 系统的调节。

更新日期：2020-05-20

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