A m6A sensing method by its impact on the stability of RNA double helix,Chemistry & Biodiversity

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A m6A sensing method by its impact on the stability of RNA double helix
Chemistry & Biodiversity ( IF 2.3 ) Pub Date : 2020-06-15 , DOI: 10.1002/cbdv.202000050
Shixi Yang ₁ , Fan Wu ₁ , Shuang Peng ₁ , Fang Wang ₂ , Yi Chen ₁ , Yushu Yuan ₁ , Xiaocheng Weng ₁ , Xiang Zhou ₁

Affiliation

N6‐Methyladenosine (m6A) is one of the most important RNA modifications in epigenetics. The development of detection method for m6A is limited by its abundance and structure. Although it has been previously reported that its presence has an impact on the complementary pairing of RNA, few assays have been developed using this finding. We used this discovery and designed a detection method based on Cas13a system, which has different fluorescence signals for target RNAs containing m6A modification and target RNAs without m6A modification. We verified the fact that the presence of m6A could cause the instability of dsRNA using the Cas13a system and provided a new direction and strategy for the development of m6A detection methods in the future.

中文翻译：

基于m6A对RNA双螺旋稳定性影响的传感方法

N6-甲基腺苷 (m6A) 是表观遗传学中最重要的 RNA 修饰之一。m6A 检测方法的发展受到其丰度和结构的限制。尽管之前有报道称其存在对 RNA 的互补配对有影响，但很少有利用这一发现开发的检测方法。我们利用这一发现设计了一种基于 Cas13a 系统的检测方法，该方法对含有 m6A 修饰的目标 RNA 和没有 m6A 修饰的目标 RNA 具有不同的荧光信号。我们使用Cas13a系统验证了m6A的存在可能导致dsRNA不稳定的事实，为未来m6A检测方法的发展提供了新的方向和策略。

更新日期：2020-06-15

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