Background: In this study, we investigate the neuroprotective effects of Hesperetin (Hst) and Nano-Hst on anxiogenic-like behavior and cerebral antioxidant defenses at transcriptional and enzymatic levels in a streptozotocin (STZ)-induced Alzheimer rat model.Methods: Wistar rats were administrated with Hst and Nano-Hst (10 and 20 mg/kg/d) for three weeks. The elevated plus-maze test assessed anxiogenic-like behavior. After behavioral test, activity and gene expression of catalase (CAT), superoxide dismutase (SOD), glutathione reductase (GRx) enzymes, as well as malondialdehyde (MDA) and glutathione (GSH) levels, were measured in the cerebral cortex.Results: Based on our results, a rat model of Alzheimer's disease (AD) exhibited anxiogenic-like behavior, activity and gene expression of cerebral antioxidant enzymes and GSH level was decreased while the MDA level was increased. Hst and Nano-Hst treatment reversed anxiogenic-like behavior, and the activities of antioxidant enzymes were elevated. Hst and Nano-Hst effects on the gene expression of CAT, SOD and GRx were confirmed by quantitative real-time PCR (qRT-PCR) in which the expression levels of these genes in the cerebral brain were significantly increased compared to STZ group.Conclusions: These findings indicated that the administration of Hst and Nano-Hst may be used to treat anxiety -related to AD via an up-regulation of cerebral antioxidant enzyme gene.

中文翻译：

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橙皮素纳米颗粒通过上调阿尔茨海默氏型痴呆中抗氧化酶的表达来减轻血管紧张素样行为和脑部氧化应激。

背景：在这项研究中，我们研究了橙皮素（Hst）和Nano-Hst在链脲佐菌素（STZ）诱导的阿尔茨海默病大鼠模型中在转录和酶促水平上对血管生成样行为和脑抗氧化剂防御的神经保护作用。用Hst和Nano-Hst（10和20 mg / kg / d）施用三周。高迷宫测试评估了类似焦虑的行为。行为测试后，在大脑皮层中测量了过氧化氢酶（CAT），超氧化物歧化酶（SOD），谷胱甘肽还原酶（GRx）酶以及丙二醛（MDA）和谷胱甘肽（GSH）的活性和基因表达。根据我们的结果，阿尔茨海默氏病（AD）的大鼠模型表现出类似焦虑的行为，脑抗氧化酶的活性和基因表达以及谷胱甘肽水平降低，而丙二醛水平升高。Hst和Nano-Hst处理可逆转类似焦虑的行为，并且抗氧化酶的活性升高。通过定量实时PCR（qRT-PCR）证实了Hst和Nano-Hst对CAT，SOD和GRx基因表达的影响，与STZ组相比，这些基因在大脑中的表达水平显着增加。 ：这些发现表明，Hst和Nano-Hst的给药可以通过上调脑抗氧化酶基因来治疗与AD相关的焦虑症。