Background: β-1, 4-galactosyltransferase III (B4GALT3) plays an important role in development tumourigenesis. But its role in human glioblastoma (GBM) is still unknown.Materials and methods: To perform gain-of-function and loss-of-function experiment, we transfected siRNA or vector into GBM cells. CCK-8, EdU staining, transwell, western blot, immunofluorescence staining were applied to perform phenotype and mechanism experiment. GlioVis platform was used to analyze B4GALT3 expression from TCGA datasets.Results: B4GALT3 is upregulated in glioblastoma and predicts poor prognosis. Upregulation of B4GALT3 promotes GBM cell proliferation and invasion while the downregulation of B4GALT3 reduces GBM cell proliferation and invasion. B4GALT3 regulates cell proliferation and invasion via β-catenin and vimentin. B4GALT3 correlates with cell invasion markers in clinical samples.Conclusion: B4GALT3 might be a potential molecular therapeutic target of glioblastoma.

中文翻译：

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B4GALT3促进胶质母细胞瘤中的细胞增殖和侵袭。

背景：β-1，4-半乳糖基转移酶III（B4GALT3）在发育肿瘤发生中起重要作用。但其在人胶质母细胞瘤（GBM）中的作用仍未知。材料与方法：为了进行功能获得和功能丧失实验，我们将siRNA或载体转染到GBM细胞中。应用CCK-8，EdU染色，transwell，western blot，免疫荧光染色进行表型和机制实验。结果表明：胶质母细胞瘤中B4GALT3表达上调，预后不良。B4GALT3的上调促进GBM细胞的增殖和侵袭，而B4GALT3的下调则减少GBM细胞的增殖和侵袭。B4GALT3通过β-连环蛋白和波形蛋白调节细胞增殖和侵袭。