Aims: The study was carried out to synthesise and characterise the chitosan-encapsulated genistein (CHI-En/Gen) and determine its anti-cancer and anti-angiogenic properties.Methods: The cytotoxic and anti-angiogenic activity of CHI-En/Gen was performed using MTT and chorioallantoic membrane assay. The molecular action was determined using flow cytometry and gene expression.Results: The synthesised CHI-En/Gen was in submicron size, spherical in shape and with entrapment efficiency and loading efficiency of 76.8% (w/w) and 32.6% (w/w), respectively. The CHI-En/Gen notably inhibited the growth and proliferation of human colorectal cancer cells (HT-29) while did not affect the viability of human dermal fibroblast as normal cell. The flow cytometry and the caspase-3 gene expression analyses revealed the apoptotic cells death in the HT-29 cells. Moreover, the encapsulated genistein showed anti-angiogenic activity.Conclusion: The CHI-En/Gen appeared as a promising carrier for the colon delivery of genistein to be used in complementary health approaches for the cancer prevention.

中文翻译：

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壳聚糖包裹的染料木黄酮的合成与表征：其抗增殖和抗血管生成活性。

目的：本研究旨在合成和表征壳聚糖包裹的染料木黄酮（CHI-En / Gen），并确定其抗癌和抗血管生成的性能。方法：CHI-En / Gen的细胞毒性和抗血管生成活性使用MTT和绒膜尿囊膜测定进行。结果：合成的CHI-En / Gen为亚微米大小，球形，包封率和载药率分别为76.8％（w / w）和32.6％（w / w）。 w）。CHI-En / Gen显着抑制人结肠直肠癌细胞（HT-29）的生长和增殖，但不影响人真皮成纤维细胞作为正常细胞的生存能力。流式细胞仪和caspase-3基因表达分析揭示了HT-29细胞中凋亡细胞的死亡。