The aim of this study is to develop a hybrid 3D printing platform integrating thermal-extrusion and electrospinning methods to fabricate bone scaffolds. The scaffolds made by mPEG–PCL–mPEG/HA biocomposite and their surface were enhanced by adding an electrospun fibre layer which improved adhesion and viability of osteoblastic cells. The scaffolds were evaluated by mechanical testing; biochemical analyses, SEM observation and their capabilities for supporting growth and adhesion of osteoblastic cells were also assessed in vitro. The 3D printing platform can manufacture the controllable and complicated shapes of bone scaffolds by controlling the thermal-extrusion and electrospinning equipment. It also can control the pore size, porosity and pore interconnectivity, and stack the scaffold structure by using different materials and different ways. Analyses showed that the bone scaffolds have a good mechanical strength and the scaffolds are suitable to support growth of MC3T3-E1 osteoblastic cells; and the electrospun fibres may increase the surface area of the fabricated scaffolds for the future application in modulating osteoblast response. Thus, it is feasible to produce bone tissue engineering scaffolds integrating thermal-extrusion and electrospinning techniques using our 3D printing platform.

这项研究的目的是开发一种结合了热挤压和静电纺丝方法来制造骨支架的混合3D打印平台。通过添加电纺纤维层可增强由mPEG–PCL–mPEG / HA生物复合材料制成的支架及其表面，从而改善成骨细胞的粘附力和生存能力。通过机械测试评估支架；还在体外评估了生化分析，SEM观察及其支持成骨细胞生长和粘附的能力。3D打印平台可以通过控制热挤压和静电纺丝设备来制造可控制的复杂形状的骨支架。它还可以控制孔的大小，孔隙率和孔的互连性，并通过使用不同的材料和不同的方式来堆叠支架结构。分析表明，该骨支架具有良好的机械强度，并且该支架适合支持MC3T3-E1成骨细胞的生长。并且电纺纤维可以增加所制造支架的表面积，以备将来在调节成骨细胞反应中的应用。因此，使用我们的3D打印平台生产结合了热挤压和静电纺丝技术的骨组织工程支架是可行的。