Industrial development has increased wastewater (WW) volume; generating contamination and disturbing ecosystems, because of breeching disposal parameters. In this work, Coloured Laboratory Wastewater (CLWW), (1500.00 colour units, CU) was separately submitted to two secondary treatments. For the first one CLWW was treated for three cycles C1, C2 and C3 with P. pastoris X33/pGAPZαA-LaccPost-Stop producing rPOXA 1B laccase, immobilized in calcium alginate beads. For the second-one, rPOXA 1B enzyme concentrate was used (three processes: P1, P2, and P3). Both treatments were carried out in a 15 L reactor with 10 L effective work volume (EWV) with 72 h hydraulic retention time. C1, C2, and C3 effluents were flocculated and filtered through quartzite sand, while P1, P2, and P3 effluents were only filtered through quartzite sand. The mixture of secondary effluents was submitted to a tertiary treatment with Chlorella sp. For C1, C2, C3, P1, P2, and P3, CU removal was of 99.16, 99.58, 99.53, 96.72, 97.05 and 96.47%, respectively. Discharge parameters, total organic carbon (TOC), inorganic carbon (IC), chemical oxygen demand (COD) and biological oxygen demand (BOD5) decreased, although they reached different final values. After the tertiary treatment (144 h) effluent discharge parameters were reduced to 34 ± 4 CU, TOC to 6.6 ± 0.9 mg L-1 and COD to 155 ± 4 mg L-1. It was demonstrated that secondary treatments (immobilized recombined cells or recombinant enzyme concentrate) combined with Chlorella sp., (tertiary treatment) attained a considerable removal of discharge parameters, demonstrating a promissory alternative for CLWW sequential treatment.

中文翻译：

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来自有色实验室废水（CLWW）的两种二次处理（固定化重组巴斯德毕赤酵母和rPOXA 1B浓缩液）的混合废水的三次处理（小球藻）。

工业发展增加了废水量。由于马裤的处理参数而产生污染并扰乱生态系统。在这项工作中，有色实验室废水（CLWW）（1500.00颜色单位，CU）分别进行了二次处理。对于第一个，将CLWW用巴斯德毕赤酵母X33 /pGAPZαA-LaccPost-Stop产生的rPOXA 1B漆酶处理3个周期C1，C2和C3，该漆酶固定在藻酸钙珠中。对于第二个，使用rPOXA 1B酶浓缩液（三个过程：P1，P2和P3）。两种处理均在15 L反应器中进行，该反应器的有效工作体积（EWV）为10 L，水力停留时间为72 h。将C1，C2和C3流出物絮凝并通过石英岩砂过滤，而P1，P2和P3流出物仅通过石英岩砂过滤。次级流出物的混合物用小球藻（Chlorella sp。）进行三次处理。对于C1，C2，C3，P1，P2和P3，CU去除率分别为99.16、99.58、99.53、96.72、97.05和96.47％。排放参数，总有机碳（TOC），无机碳（IC），化学需氧量（COD）和生物需氧量（BOD5）降低，尽管它们达到了不同的最终值。经过三次处理（144小时）后，出水参数降至34±4 CU，TOC降至6.6±0.9 mg L-1，COD降至155±4 mg L-1。结果表明，与小球藻结合的第二种处理方法（固定化的重组细胞或重组酶浓缩物）（第三种处理方法）可实现相当大的排放参数去除，这证明了CLWW顺序处理方法的替代方案。