A new simple analytical method was developed for the identification and quantification of aloin in aloe gel. The extraction was performed using acetonitrile acidified with formic acid (1%, v/v) with no additional cleanup step. The compound was determined using high-performance liquid chromatography coupled with triple quadrupole mass spectrometry (QqQ). The recovery rates obtained were between 80 and 110% with a relative standard deviation < 26%, at four different concentration levels. The calculated limit of quantification (LOQ) was at 0.05 mg kg⁻¹.

中文翻译：

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液相色谱串联质谱法测定芦荟苷分析方法的建立与验证

开发了一种新的简单分析方法，用于芦荟凝胶中芦荟苷的鉴定和定量。使用用甲酸（1％，v / v）酸化的乙腈进行萃取，无需额外的纯化步骤。使用高效液相色谱结合三重四极杆质谱（QqQ）测定该化合物。在四个不同的浓度水平下，回收率在80％至110％之间，相对标准偏差<26％。计算的定量极限（LOQ）为0.05mg kg ^-1。