Background Mesenchymal Stem Cells (MSCs), regardless of the tissue sources, are considered as excellent candidates for cellular therapy as they are immune-privileged cells containing a multitude of therapeutic functions that aid in tissue regeneration and repair. For the effective application of these cells in cell therapy, it is important to understand and characterize their biological functions. Objectives The present study attempts to characterize the variations in multipotent function such as cell surface antigen levels, proliferation, differentiation and stemness (pluripotency) potential of MSCs isolated from foetal [wharton’s jelly (WJ), foetal and maternal side of placenta (PF and PM)] and adult tissue sources [bone marrow (BM) and adipose tissue (AT)] using gene expression by real time PCR (qRT-PCR). Results Amongst the different tissue sources, PM, PF and AT-MSCs exhibited significant increase (p < 0.001, p < 0.001 and p < 0.01 respectively) in CD 73 expression and therefore could have a role in immunomodulation. WJ-MSCs exhibited superior proliferation potential based on growth curve, PCNA and Wnt gene expression. BM-MSCs were superior in exhibiting trilineage differentiation. Enhanced stemness potential (Oct 4 and Nanog) was observed for both BM and WJ-MSCs. In addition, BM and WJ-MSCs expressed high levels of CD 90 making them suitable in bone repair and regeneration. Conclusion Thus to conclude, out of the five different sources tested, BM an adult source and WJ-MSCs a foetal source were superior in exhibiting most of the biological functions indicating that these sources may be suitable candidates for cell repair and regeneration studies.

中文翻译：

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不同组织来源间充质干细胞之间的功能差异：比较基因表达谱

背景 间充质干细胞 (MSCs)，无论组织来源如何，都被认为是细胞治疗的绝佳候选者，因为它们是具有免疫特权的细胞，包含多种有助于组织再生和修复的治疗功能。对于这些细胞在细胞治疗中的有效应用，了解和表征它们的生物学功能非常重要。目的 本研究试图表征从胎儿 [沃顿氏胶 (WJ)、胎儿和母体胎盘 (PF 和 PM) 分离的 MSC 的多能功能变化，例如细胞表面抗原水平、增殖、分化和干细胞 (多能性) 潜能。 )] 和成人组织来源 [骨髓 (BM) 和脂肪组织 (AT)] 使用实时 PCR (qRT-PCR) 的基因表达。结果 在不同组织来源中，PM、PF 和 AT-MSCs 的 CD 73 表达显着增加（分别为 p < 0.001、p < 0.001 和 p < 0.01），因此可能在免疫调节中起作用。基于生长曲线、PCNA 和 Wnt 基因表达，WJ-MSCs 表现出优越的增殖潜力。BM-MSCs 表现出三系分化的优势。BM 和 WJ-MSC 均观察到增强的干细胞潜能（10 月 4 日和 Nanog）。此外，BM 和 WJ-MSC 表达高水平的 CD 90，使其适用于骨修复和再生。结论 因此得出的结论是，在测试的五个不同来源中，