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Garlic essential oil reduces the population of Meloidogyne incognita in tomato plants
European Journal of Plant Pathology ( IF 1.7 ) Pub Date : 2020-05-06 , DOI: 10.1007/s10658-020-02000-1
I. N. Jardim , D. F. Oliveira , V. P. Campos , G. H. Silva , P. E. Souza

The root-knot nematode Meloidogyne incognita causes losses in tomato production, making new methods/products to control this parasite desirable. Garlic essential oil (GEO) is potentially useful for controlling M. incognita. Here, GEO was obtained by hydrodistillation, dissolved in water and used against M. incognita in vitro. At 63 μg mL−1, this oil was more active against M. incognita eggs and second-stage juveniles (J2) than the nematicide Carbofuran at 173 μg mL−1. The main components of the oil, according to gas chromatography-mass spectrometry analysis, are diallyl trisulphide (66.7%) and diallyl disulphide (21.3%), which presented lethal concentrations to 50% (LC50) J2 equal to 36.2 ± 1.7 and 134.4 ± 19.4 μg mL−1, respectively, while the LC50 for Carbofuran was 151.6 ± 2.1 μg mL−1. When the J2 submerged in a solution of the oil at 250 μg mL−1 were used to infest tomato plants, the number of galls and eggs of M. incognita in the roots were reduced to values statistically equal to those obtained when J2 were submerged in a solution of Carbofuran at 415 μg mL−1. The vapour of the oil was also as active in vitro against M. incognita eggs and J2 as the fumigant nematicide Dazomet. The infectivity and reproduction of M. incognita in tomato plants cultivated in substrate inoculated with eggs of the nematode and treated with 0.2 mL of oil per L of substrate were statistically equal to those observed when the oil was replaced by 0.25 g of Dazomet per L of substrate. These findings confirm the activity of GEO and its components against M. incognita, suggesting its potential as a new fumigant nematicide to control the nematode in tomato plants.



中文翻译:

大蒜精油减少了番茄植株中根结线虫的数量

根节线虫南方根结线虫造成损失在番茄生产,使得新方法/产品来控制这种寄生虫期望的。大蒜精油(GEO)对于控制隐身支原体可能很有用。这里,GEO通过水蒸气蒸馏得到的,溶解在水中并用抗南方根结线虫体外。在63μgmL -1时,此油比在173μgmL -1时使用杀线虫剂呋喃呋喃具有更高的抗隐线虫卵和二级幼虫(J2)的活性。根据气相色谱-质谱分析,该油的主要成分是三烯丙基二硫化物(66.7%)和二烯丙基二硫化物(21.3%),其致死浓度为50%(LC)。50)J2分别等于36.2±1.7和134.4±19.4μgmL -1,而呋喃丹的LC 50为151.6±2.1μgmL -1。当使用浸没在250μgmL -1的油溶液中的J2侵染番茄植株时,根部隐身梭形虫的gall虫和卵的数量减少到统计学上等于J2浸没在番茄中的值。呋喃丹溶液415μgmL -1。油的蒸气也作为活性体外抗南方根结线虫卵和J2作为熏蒸剂杀线虫剂棉隆。隐孢子虫的感染性和繁殖在番茄植物中种植的线虫卵接种了底物,每升底物用0.2 mL油处理的番茄植株,在统计学上等于每升底物用0.25 g Dazomet代替油时所观察到的番茄植株。这些发现证实了GEO及其组分对隐身梭状芽胞杆菌的活性,表明其作为控制番茄植物线虫的新型熏蒸杀线虫剂的潜力。

更新日期:2020-05-06
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