Airborne bacteria and fungi in hospital environments are of great concern due to their potential role as a source of nosocomial infections. The aim of this study was to evaluate concentration and diversity of airborne bacteria and fungi in relation to particle mass concentration in sensitive wards of a pediatric hospital. The study was performed in the cardiac care unit (CCU), the neonatal intensive care unit (NICU), the cancer blood ward (BW), the ENT (ear, nose, throat) operation room (OT1) and the eye operation room (OT2). The air samples were collected by impaction using the single-stage Andersen sampler. The flow rate and sampling time of the pump were adjusted to 28.3 l/min for 5 min. The mean concentration of indoor airborne fungi and bacteria ranged from 0–63 to 19–356 CFU/m3, respectively. OT2 and CCU wards were the most contaminated wards for airborne bacteria and fungi, respectively (243 ± 77 vs. 30 ± 7 CFU/m3). The airborne Gram-positive cocci ( Staphylococcus and Micrococcus ) were the most detected bacterial genera (75%) in all indoor air samples, and the most prevalent genera in indoor environment were Cladosporium spp. (19%) followed by Penicillium spp. (16%), Aspergillus spp. (16%) and Paecilomyces spp. (10%). Results showed that the outdoor airborne bacteria and PM concentration at different sizes were significantly higher than indoors, suggesting that the indoor airborne particle may have originated from the outdoor air. There were significant positive relationships between indoor airborne fungi concentrations with indoor PM 2.5 and PM 10 .

中文翻译：

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学术儿科医院敏感病房空气传播细菌和真菌的室内暴露

医院环境中的空气传播细菌和真菌因其作为医院感染源的潜在作用而备受关注。本研究的目的是评估空气传播细菌和真菌的浓度和多样性与儿科医院敏感病房中颗粒质量浓度的关系。研究在心脏监护病房（CCU）、新生儿重症监护病房（NICU）、癌症血病病房（BW）、耳鼻喉科手术室（OT1）和眼科手术室（ OT2）。使用单级安德森采样器通过撞击收集空气样本。将泵的流速和采样时间调整为 28.3 l/min，持续 5 分钟。室内空气中真菌和细菌的平均浓度分别为 0-63 至 19-356 CFU/m3。OT2 和 CCU 病房分别是空气传播细菌和真菌污染最严重的病房（243 ± 77 vs. 30 ± 7 CFU/m3）。空气中革兰氏阳性球菌（葡萄球菌和微球菌）是所有室内空气样本中检出率最高的细菌属（75%），室内环境中最普遍的属是枝孢菌属。(19%) 其次是青霉属。(16%)，曲霉属。(16%) 和拟青霉属。(10%)。结果表明，室外空气中不同大小的细菌和PM浓度均显着高于室内，表明室内空气中的颗粒可能来源于室外空气。室内空气中真菌浓度与室内PM 2.5 和PM 10 之间存在显着的正相关关系。空气中革兰氏阳性球菌（葡萄球菌和微球菌）是所有室内空气样本中检出率最高的细菌属（75%），室内环境中最普遍的属是枝孢菌属。(19%) 其次是青霉属。(16%)，曲霉属。(16%) 和拟青霉属。(10%)。结果表明，室外空气中不同大小的细菌和PM浓度均显着高于室内，表明室内空气中的颗粒可能来源于室外空气。室内空气中真菌浓度与室内PM 2.5 和PM 10 之间存在显着的正相关关系。空气中革兰氏阳性球菌（葡萄球菌和微球菌）是所有室内空气样本中检出率最高的细菌属（75%），室内环境中最普遍的属是枝孢菌属。(19%) 其次是青霉属。(16%)，曲霉属。(16%) 和拟青霉属。(10%)。结果表明，室外空气中不同大小的细菌和PM浓度均显着高于室内，表明室内空气中的颗粒可能来源于室外空气。室内空气中真菌浓度与室内PM 2.5 和PM 10 之间存在显着的正相关关系。(19%) 其次是青霉属。(16%)，曲霉属。(16%) 和拟青霉属。(10%)。结果表明，室外空气中不同大小的细菌和PM浓度均显着高于室内，表明室内空气中的颗粒可能来源于室外空气。室内空气中真菌浓度与室内PM 2.5 和PM 10 之间存在显着的正相关关系。(19%) 其次是青霉属。(16%)，曲霉属。(16%) 和拟青霉属。(10%)。结果表明，室外空气中不同大小的细菌和PM浓度均显着高于室内，表明室内空气中的颗粒可能来源于室外空气。室内空气中真菌浓度与室内PM 2.5 和PM 10 之间存在显着的正相关关系。