In order to facilitate direct testing of the biological effects of radon, we designed and constructed a 3.1 m³ radon chamber specifically for radon exposures to small animals. The chamber is designed to operate as a sealed enclosure with a controlled atmosphere containing a known concentration of radon and its radioactive decay products. Sensors for air flow rate, temperature, humidity, HEPA filter and differential pressure ensure an optimal environment for exposure subjects. The radon gas is supplied to the chamber from a generator containing Radium-226 in a dilute acid solution. Air containing radon can be pumped continuously using a constant flow rate to maintain a steady state supply. The source flow rate was partitioned to achieve a chamber concentration at 200 Bq/m³ (R² = 0.9341) or 1000 Bq/m³ (R² = 0.9715). Small particles are injected into the re-circulating air stream via a particle generator to provide condensation nuclei for attachment of radon decay products as they form in the chamber atmosphere. Particles measured at 0.3 μm, 0.5 μm and 5.0 μm averaged concentrations 5.7 ± 0.6 × 10⁷/m³, 2.5 ± 0.7 × 10⁷/m³ and 2.3 ± 2.4 × 10³/m³, respectively. A desired Equilibrium Factor can easily be achieved by varying the air circulation rate through the chamber. The Equilibrium Factor ranged from 0.4 to 0.8 at 200 Bq/m³ and 0.5 to0.6 at 1000 Bq/m³. The chamber was designed to conduct short term exposures to assess the acute cellular changes induced by radon exposure. To our knowledge, this is currently the only radon chamber designed specifically to investigate environmentally relevant exposure time and doses of radon gas and decay products in small animal models.

为了便于直接测试of的生物学效应，我们设计并建造了一个3.1 m ^3的chamber室，专门用于暴露于小动物的don。该腔室设计为在受控气氛中作为密封外壳运行，该受控气氛包含已知浓度的ra及其放射性衰变产物。空气流速，温度，湿度，HEPA过滤器和压差传感器确保了暴露对象的最佳环境。containing气是从稀酸溶液中含有Radium-226的发生器供应到燃烧室的。可以使用恒定流速连续泵送含ra空气，以维持稳定状态的供气。分配源流速以达到200 Bq / m ³（R²  = 0.9341）或1000 Bq / m ³（R ²  = 0.9715）。小颗粒通过颗粒发生器注入到再循环空气流中，以提供凝结核，以便在腔室内形成decay衰变产物时将其附着。粒子在0.3μm的测定，在0.5μm和5.0μm的平均浓度5.7±0.6×10 ⁷ /米³，2.5±0.7×10 ⁷ /米³和2.3±2.4×10 ³ /米³分别。通过改变通过腔室的空气循环速率可以容易地获得所需的平衡因数。平衡因子在200 Bq / m ^3时从0.4到0.8到1000 Bq / m ^3时在0.5到0.6的范围内。该室设计用于进行短期暴露以评估ra暴露引起的急性细胞变化。据我们所知，这是目前唯一专门设计用于调查与环境有关的暴露时间以及小动物模型中ra气和衰变产物剂量的ra室。