A four-week white bread diet does not alter plasma glucose concentrations, metabolic or vascular physiology in mourning doves, Zenaida macroura.,Comparative Biochemistry and Physiology A: Molecular & Integrative Physiology

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A four-week white bread diet does not alter plasma glucose concentrations, metabolic or vascular physiology in mourning doves, Zenaida macroura.
Comparative Biochemistry and Physiology A: Molecular & Integrative Physiology ( IF 2.1 ) Pub Date : 2020-05-03 , DOI: 10.1016/j.cbpa.2020.110718
Anthony J Basile ₁ , Paniz Jasbi ₂ , William Clark ₁ , Xiaojian Shi ₂ , Haiwei Gu ₂ , Pierre Deviche ₁ , Karen L Sweazea ₃

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Birds are an enigma: their plasma glucose concentration is 1.5-2 times higher than similar-sized mammals, yet they do not normally exhibit symptoms of diabetes. We hypothesized that feeding adult mourning doves a refined carbohydrate diet (white bread: WB) for four weeks would raise plasma glucose concentrations and alter metabolic pathways and endothelial function when compared to birds receiving a nutritionally-balanced diet (bird seeds: SD). Following the four-week long diets, birds were euthanized, and cardiac blood, liver, and pectoralis muscles were collected for metabolomics analyses and biochemical assays. Cranial tibial arteries were dissected to measure acetylcholine-mediated vasodilation. Contrary to the hypothesis, WB-fed birds did not have increased plasma glucose concentrations. Principle component analysis score plots suggest minimal differences between groups. However, we identified 15 changes in individual metabolite concentrations between diet groups that, although not statistically significant, are highly predictive (area under receive operating curve, AUROC>0.90; number of highly predictive metabolites: 5 of 123 in plasma, 4 of 92 in liver, and 6 of 92 in pectoralis muscle). Moreover, pathway analyses revealed no significantly altered metabolic pathways between groups. Biochemical assays revealed no significant group differences in plasma uric acid and insulin, or pectoralis muscle glycogen concentrations. However, hepatic glycogen concentration was 2.12-fold higher in the WB group than in control doves (p = .015). Diet type did not influence vasodilation. In conclusion, a four-week long white bread diet increased liver glycogen but did not alter plasma glucose concentrations, metabolic or vascular physiology in mourning doves.

中文翻译：

为期四周的白面包饮食不会改变哀悼鸽子Zenaida macroura的血浆葡萄糖浓度，代谢或血管生理。

鸟类是一个谜：它们的血浆葡萄糖浓度比类似大小的哺乳动物高1.5-2倍，但它们通常不会表现出糖尿病症状。我们假设，与接受营养均衡饮食的鸟类（鸟类种子）相比，喂成年哀悼鸽子精制碳水化合物的饮食（白面包：WB）会提高血浆葡萄糖浓度，并改变代谢途径和内皮功能。经过四周的饮食，安乐死了家禽，并收集了心脏血液，肝脏和胸大肌进行代谢组学分析和生化分析。解剖颅胫动脉以测量乙酰胆碱介导的血管舒张作用。与该假设相反，WB喂养的家禽的血浆葡萄糖浓度没有升高。主成分分析得分图显示了组之间的最小差异。但是，我们确定了饮食组之间个体代谢物浓度的15种变化，尽管这些变化在统计学上不显着，但具有高度预测性（接收操作曲线下的区域，AUROC> 0.90；高度预测性代谢物的数量：血浆中123种中有5种，92种中有4种在血浆中肝，以及胸大肌中92个中的6个）。此外，途径分析显示组之间的代谢途径没有显着改变。生化分析显示血浆尿酸和胰岛素或胸大肌糖原浓度无明显差异。但是，WB组的肝糖原浓度比对照组的鸽子高2.12倍（p = .015）。饮食类型不影响血管舒张。结论，

更新日期：2020-05-05

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