Embryos (n = 87) collected 8 days after mating and 7 days after ovulation were vitrified using a camel-specific vitrification kit. Vitrification solutions (VS) containing 20% foetal calf serum, with or without 2% bovine serum albumin (BSA) were used to cryopreserve embryos, in three steps VS1 (5 min), VS2 (5 min) and VS3 (30−35 s) at room temperature (RT) before being loaded into open pulled straws and immediately frozen in liquid nitrogen. Embryos were subsequently thawed in warming solutions (WS) in three steps: WS1 at 37 °C (1 min), WS2 at RT (5 min) then into holding media at RT (5−60 min) prior to transfer, in pairs, into recipient camels 6 days after ovulation. There were 42 of 43 embryos viable after vitrification in media without BSA and these were subsequently transferred into 21 recipient females which resulted in ten pregnancies 60 days after transfer (48% pregnancy rate). There were 38 of 44 viable embryos vitrified in media containing BSA that were transferred in pairs into 19 recipient females which resulted in five pregnancies 60 days after transfer (26% pregnancy rate; P > 0.05). Of the total 15 foetuses that developed to 60 days of gestation after vitrification, 11 resulted from embryos of 200–499 μm diameter and four from embryos of 500–700 μm diameter (P > 0.05). There were encouraging results with use of this novel vitrification kit for the commercial application of cryopreservation of camel embryos with a diameter of 300–550 μm.

胚胎（ñ （＝ 87）在交配后8天和排卵后7天使用骆驼特异性玻璃化试剂盒对玻璃化。含20％胎牛血清，有或没有2％牛血清白蛋白（BSA）的玻璃化溶液（VS）分三个步骤VS1（5分钟），VS2（5分钟）和VS3（30-35 s）用于冷冻保存胚胎），然后在室温（RT）下装入敞开的拉管中，并立即在液氮中冷冻。随后分三步将胚胎在温热溶液（WS）中解冻：在37°C（1分钟）的WS1，在RT（5分钟）的WS2然后在RT（5-60分钟）的条件下成对转移到保持培养基中排卵后6天进入受体骆驼。在没有BSA的培养基中玻璃化后可存活的43个胚胎中有42个，随后将它们移植到21个接受雌性的雌性中，导致移植后60天十次怀孕（妊娠率48％）。在含有BSA的培养基中玻璃化的44个有生命的胚胎中，有38个被成对地转移到19名接受雌性的雌性中，导致在转移60天后进行了5次怀孕（妊娠率为26％；P > 0.05）。在玻璃化后发展到妊娠60天的15胎中，有11胎是直径200-499μm的胚胎，有4胎是直径500-700μm的胚胎（P> 0.05）。将该新型玻璃化试剂盒用于冷冻保存直径为300–550μm的骆驼胚胎的商业应用，取得了令人鼓舞的结果。