To overcome the serious shortage of donor corneas for transplantation, alternatives based on tissue engineering need to be developed. Decellularized corneas are one potential alternative, but their densely packed collagen architecture inhibits recellularization in vitro. Therefore, a new rapid method of recellularizing these constructs to ensure high cellularity throughout the collagen scaffold is needed. In this study, we developed a novel method for fabricating corneal constructs by using decellularized porcine corneal sheets assembled using a bottom-up approach by layering multiple sheets between cell-laden collagen I hydrogel.

中文翻译：

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使用新型多层制造组装技术设计角膜基质等效物。

为了克服用于移植的供体角膜的严重短缺，需要开发基于组织工程的替代方案。去细胞角膜是一种潜在的替代方法，但其密集的胶原结构会抑制体外再细胞化。因此，需要一种新的快速重新细胞化这些构建体的方法，以确保整个胶原支架的高细胞性。在这项研究中，我们开发了一种新的角膜结构制造方法，该方法使用自下而上的方法组装的脱细胞猪角膜片，通过在载有细胞的 I 型胶原水凝胶之间分层多层片。