Abstract Microbial cells can rapidly form biofilm on endotracheal tubes (ETT) causing ventilator-associated pneumonia, a serious complication in patients receiving mechanical ventilation. A novel polyamide with a good balance of hydrophilic/hydrophobic moieties was used for the embedment of green-reduction silver nanoparticles (AgNPs) for the composite-coated ETT. The films were conformal with a thickness of ∼ 17 ± 3 µm accommodating high loading of 60 ± 35 nm spherical-shaped AgNPs. The coated ETT resulted in a significant difference in reducing both planktonic growth and microbial adhesion of single and mixed-species cultures, compared with uncoated ETT (p < 0.05). A time-kill assay demonstrated rapid bactericidal effects of the coating on bacterial growth and cell adhesion to ETT surface. Biofilm formation by Pseudomonas aeruginosa and Staphylococcus aureus, commonly encountered pathogens, was inhibited by > 96% after incubation for 72 h. Polyamide/AgNP composite-coated ETT provided a broad-spectrum activity against both Gram-positive and Gram-negative bacteria as well as Candida albicans and prolonged antimicrobial activity.

中文翻译：

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使用新型聚酰胺/银纳米颗粒复合涂层气管导管对引起呼吸机相关性肺炎的病原体的浮游生长、粘附和生物膜形成具有长期抑制作用

摘要 微生物细胞可在气管插管 (ETT) 上迅速形成生物膜，导致呼吸机相关性肺炎，这是机械通气患者的严重并发症。具有亲水/疏水部分良好平衡的新型聚酰胺用于嵌入复合涂层 ETT 的绿色还原银纳米粒子 (AgNPs)。薄膜保形，厚度约为 17 ± 3 µm，可容纳 60 ± 35 nm 球形 AgNPs 的高负载。与未包被的 ETT 相比，包被的 ETT 在减少单一和混合物种培养物的浮游生长和微生物粘附方面产生显着差异（p < 0.05）。时间杀灭试验证明了涂层对细菌生长和细胞粘附到 ETT 表面的快速杀菌作用。铜绿假单胞菌和金黄色葡萄球菌（常见的病原体）的生物膜形成在孵育 72 小时后被抑制 > 96%。聚酰胺/AgNP 复合涂层 ETT 提供了针对革兰氏阳性和革兰氏阴性细菌以及白色念珠菌的广谱活性和延长的抗菌活性。