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Column-free purification and coating of SpyCatcher protein on ELISA wells generates universal solid support for capturing of SpyTag-fusion protein from the non-purified condition.
Protein Expression and Purification ( IF 1.6 ) Pub Date : 2020-04-29 , DOI: 10.1016/j.pep.2020.105650 Mohammad Karimi Baba Ahmadi 1 , Seyed Abolghasem Mohammadi 2 , Manoochehr Makvandi 3 , Morteza Mamoueie 4 , Mohammad Rahmati 5 , David Wood 6
Protein Expression and Purification ( IF 1.6 ) Pub Date : 2020-04-29 , DOI: 10.1016/j.pep.2020.105650 Mohammad Karimi Baba Ahmadi 1 , Seyed Abolghasem Mohammadi 2 , Manoochehr Makvandi 3 , Morteza Mamoueie 4 , Mohammad Rahmati 5 , David Wood 6
Affiliation
•Spy Tag-Protein covalent interaction is rapid and specific method for protein immobilization.•Column free purification of SpyCatcher protein enables develop a universal solid support for SpyTag protein purification.•This method is highly simple and applicable to other proteins.
中文翻译：
ELISA孔上无柱纯化和SpyCatcher蛋白包被产生通用固相支持物，用于从非纯化条件下捕获SpyTag-fusion蛋白。
•Spy Tag-Protein共价相互作用是固定蛋白质的快速而特定的方法。•SpyCatcher蛋白的无柱纯化可为SpyTag蛋白的纯化开发通用的固体支持物。•此方法非常简单，适用于其他蛋白质。
更新日期：2020-04-28
中文翻译：
ELISA孔上无柱纯化和SpyCatcher蛋白包被产生通用固相支持物，用于从非纯化条件下捕获SpyTag-fusion蛋白。
•Spy Tag-Protein共价相互作用是固定蛋白质的快速而特定的方法。•SpyCatcher蛋白的无柱纯化可为SpyTag蛋白的纯化开发通用的固体支持物。•此方法非常简单，适用于其他蛋白质。
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