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A basic ddRADseq two-enzyme protocol performs well with herbarium and silica-dried tissues across four genera.
Applications in Plant Sciences ( IF 2.7 ) Pub Date : 2020-04-23 , DOI: 10.1002/aps3.11344
Ingrid E Jordon-Thaden 1, 2 , James B Beck 3, 4 , Catherine A Rushworth 5, 6 , Michael D Windham 7 , Nicolas Diaz 8, 9 , Jason T Cantley 8, 10 , Christopher T Martine 8 , Carl J Rothfels 1
Affiliation  

The ability to sequence genome‐scale data from herbarium specimens would allow for the economical development of data sets with broad taxonomic and geographic sampling that would otherwise not be possible. Here, we evaluate the utility of a basic double‐digest restriction site–associated DNA sequencing (ddRADseq) protocol using DNAs from four genera extracted from both silica‐dried and herbarium tissue.

中文翻译:


基本的 ddRADseq 双酶方案对四个属的植物标本室和二氧化硅干燥组织表现良好。



对植物标本的基因组规模数据进行测序的能力将允许经济地开发具有广泛的分类学和地理采样的数据集,否则这是不可能的。在这里,我们使用从二氧化硅干燥和植物标本中提取的四个属的 DNA 来评估基本双消化限制性位点相关 DNA 测序 (ddRADseq) 方案的实用性。
更新日期:2020-04-23
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