Nesfatin-1 is a multifunctional neuropeptide having crucial autonomic roles. It is well known that nesfatin-1 collaborates with other central neuromodulatory systems, such as central corticotropin-releasing hormone, melanocortin, oxytocin, and cholinergic systems to show its autonomic effects. Central arachidonic acid cascade plays an important role to provide the homeostasis by exhibiting similar autonomic effects to nesfatin-1. Based on these similarities, the current study was designed to show the effects of intracerebroventricularly (ICV) injected nesfatin-1 on the hypothalamic arachidonic acid (AA) cascade. Immunochemistry and western blot approaches demonstrated that ICV administration of nesfatin-1 provokes an increase in the hypothalamic cyclooxygenase (COX) -1, -2 and lipoxygenase (LOX) protein expression. Moreover, the microdialysis study demonstrated that centrally injected nesfatin-1 increased the posterior hypothalamic extracellular AA products. In conclusion, these findings report that while nesfatin-1 is generating its autonomic effects, it also might be using central prostaglandins and leukotrienes by activating central COX and LOX pathways.

中文翻译：

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脑室内注射 nesfatin-1 激活中枢环氧化酶和脂肪氧化酶通路

Nesfatin-1 是一种多功能神经肽，具有重要的自主神经作用。众所周知，nesfatin-1 与其他中枢神经调节系统合作，如中枢促肾上腺皮质激素释放激素、黑皮质素、催产素和胆碱能系统，以显示其自主神经作用。中央花生四烯酸级联通过表现出与 nesfatin-1 相似的自主神经效应，在提供体内平衡方面发挥着重要作用。基于这些相似之处，本研究旨在显示脑室内 (ICV) 注射 nesfatin-1 对下丘脑花生四烯酸 (AA) 级联反应的影响。免疫化学和蛋白质印迹方法表明，nesfatin-1 的 ICV 给药会引起下丘脑环氧化酶 (COX) -1、-2 和脂肪氧化酶 (LOX) 蛋白表达的增加。而且，微透析研究表明，中央注射 nesfatin-1 增加了下丘脑后部细胞外 AA 产物。总之，这些发现表明，虽然 nesfatin-1 正在产生其自主神经效应，但它也可能通过激活中枢 COX 和 LOX 通路来使用中枢前列腺素和白三烯。