Background: Colorectal cancer (CRC) represents a third leading cause of cancer-related death worldwide. The reliable diagnostic biomarkers for detecting CRC at early stage is critical for decreasing the mortality.Method: A conjunctive lipidomic approach was employed to investigate the differences in plasma lipid profiles of CRC patients (n = 101) and healthy volunteers (n = 52). Based on UHPLC-Q-TOF MS and UHPLC-QQQ MS platforms, a total of 236 lipids were structurally detected. Multivariate data analysis was conducted for biomarkers discovery.Results: A total of 11 lipid species, including 1 Glycerophosphoethanolamine (PE), 3 ethanolamine plasmalogens (PlsEtn), 1 plasmanyl glycerophosphatidylethanolamine (PE-O), 3 fatty acids (FFA), 1 Fatty acid ester of hydroxyl fatty acid (FAHFA) and 2 Diacylglycerophosphates (PA) were identified to distinguish the CRC patients at early stage from healthy controls. In addition, these potential lipid biomarkers achieved an estimated AUC=0.981 in a validation set for univariate ROC analysis.Conclusion: By combining Q-TOF MS and QQQ MS analysis, the 11 lipids exhibited good performance in differentiating early-stage CRC and healthy control. This study also demonstrated that lipidomics is a powerful tool in discovering new potential biomarkers for cancer diagnosis.

中文翻译：

---

联合脂质组学方法揭示了血浆乙醇胺缩醛磷脂和脂肪酸作为结直肠癌患者的早期诊断生物标志物。

背景：结直肠癌 (CRC) 是全球癌症相关死亡的第三大原因。早期检测CRC的可靠诊断生物标志物对于降低死亡率至关重要。方法：采用联合脂质组学方法研究CRC患者（n = 101）和健康志愿者（n = 52）血浆脂质谱的差异。基于 UHPLC-Q-TOF MS 和 UHPLC-QQQ MS 平台，共检测到 236 种脂质。对生物标志物的发现进行了多变量数据分析。 结果：共 11 种脂质，包括 1 种甘油磷酸乙醇胺 (PE)、3 种乙醇胺缩醛磷脂 (PlsEtn)、1 种血浆甘油磷脂酰乙醇胺 (PE-O)、3 种脂肪酸 (FFA)、确定了 1 种羟基脂肪酸脂肪酸酯 (FAHFA) 和 2 种二酰基甘油磷酸酯 (PA)，以区分早期 CRC 患者与健康对照。此外，这些潜在的脂质生物标志物在单变量 ROC 分析的验证集中达到了估计的 AUC=0.981。 结论：通过结合 Q-TOF MS 和 QQQ MS 分析，11 种脂质在区分早期 CRC 和健康对照方面表现出良好的性能. 该研究还表明，脂质组学是发现用于癌症诊断的新潜在生物标志物的有力工具。这11种脂质在区分早期CRC和健康对照方面表现出良好的性能。该研究还表明，脂质组学是发现用于癌症诊断的新潜在生物标志物的有力工具。这11种脂质在区分早期CRC和健康对照方面表现出良好的性能。该研究还表明，脂质组学是发现用于癌症诊断的新潜在生物标志物的有力工具。